Change search
ReferencesLink to record
Permanent link

Direct link
Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance
Umeå University, Faculty of Science and Technology, Chemistry.
Show others and affiliations
2009 (English)In: Journal of Cell Biology, ISSN 0021-9525, E-ISSN 1540-8140, Vol. 185, no 3, 475-91 p.Article in journal (Refereed) Published
Abstract [en]

The nuclear pore complex (NPC) mediates all nucleocytoplasmic transport, yet its structure and biogenesis remain poorly understood. In this study, we have functionally characterized interaction partners of the yeast transmembrane nucleoporin Ndc1. Ndc1 forms a distinct complex with the transmembrane proteins Pom152 and Pom34 and two alternative complexes with the soluble nucleoporins Nup53 and Nup59, which in turn bind to Nup170 and Nup157. The transmembrane and soluble Ndc1-binding partners have redundant functions at the NPC, and disruption of both groups of interactions causes defects in Ndc1 targeting and in NPC structure accompanied by significant pore dilation. Using photoconvertible fluorescent protein fusions, we further show that the depletion of Pom34 in cells that lack NUP53 and NUP59 blocks new NPC assembly and leads to the reversible accumulation of newly made nucleoporins in cytoplasmic foci. Therefore, Ndc1 together with its interaction partners are collectively essential for the biosynthesis and structural integrity of yeast NPCs.

Place, publisher, year, edition, pages
The Rockefeller University Press , 2009. Vol. 185, no 3, 475-91 p.
URN: urn:nbn:se:umu:diva-23148DOI: 10.1083/jcb.200810030OAI: diva2:220533
Available from: 2009-06-01 Created: 2009-06-01 Last updated: 2010-04-15Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textExtern länk

Search in DiVA

By author/editor
Kieselbach, Thomas
By organisation
In the same journal
Journal of Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 30 hits
ReferencesLink to record
Permanent link

Direct link