High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas
2008 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 13, no 5, 054070- p.Article in journal (Refereed) Published
A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.
Place, publisher, year, edition, pages
Bellingham, WA: SPIE--the International Society for Optical Engineering , 2008. Vol. 13, no 5, 054070- p.
biological organs, biomedical optical imaging, cellular biophysics, diseases, image resolution, laser applications in medicine, molecular biophysics, optical tomography, optical projection tomography, confocal microscopy, pancreas, microbiopsy, insulitis, diabetes
IdentifiersURN: urn:nbn:se:umu:diva-23351DOI: 10.1117/1.3000430PubMedID: 19021448OAI: oai:DiVA.org:umu-23351DiVA: diva2:223435