The role of chemokines and extracellular matrix components in the migration of T lymphocytes into three-dimensional substrata.
2005 (English)In: Immunology, ISSN 0019-2805, Vol. 114, no 1, 53-62 p.Article in journal (Refereed) Published
The role of chemokines and their interactions with extracellular matrix components (ECM) or the capacity of T cells to migrate into and accumulate within three-dimensional (3D) collagen type 1 substrata was studied. We examined the influence of chemokines and fibronectin on the infiltration properties of non-infiltrative (do not migrate into 3D substrata) and spontaneously infiltrative (migrate into 3D substrata) T-cell lines. Infiltrative and non-infiltrative T-acute lymphocytic leukaemic cell lines exhibited no consistent differences with respect to the expression of various chemokine receptors or beta(1)-integrins. Chemokines presented inside the collagen increased the depth of migration of infiltrative T-cell lines, but did not render non-infiltrative T-cell lines infiltrative, although they augmented the attachment of non-infiltrative T-cell lines to the upper surface of the collagen. The presence of fibronectin inside the collagen did not render non-infiltrative T-cell lines infiltrative, but markedly augmented the migration of 'infiltrative' T-cell lines into collagen. Both infiltrative and non-infiltrative T-cell lines showed migratory responses to chemokines in Boyden assays (migration detected on 2D substrata). These results indicate that the process of T-cell infiltration/migration into 3D substrata depends on a tissue penetration mechanism distinguishable from migration on 2D substrata and that the basic capacity of T cells to infiltrate is independent of chemokines and ECM components applied as attractants.
Place, publisher, year, edition, pages
2005. Vol. 114, no 1, 53-62 p.
IdentifiersURN: urn:nbn:se:umu:diva-23372DOI: 10.1111/j.1365-2567.2004.02005.xPubMedID: 15606795OAI: oai:DiVA.org:umu-23372DiVA: diva2:223635