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Nestin is expressed in mesenchymal and not epithelial cells of the developing mouse pancreas
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). (Helena Edlund)
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). (Helena Edlund)
2002 (English)In: Mechanisms of Development, ISSN 0925-4773, Vol. 113, no 2, 189-192 p.Article in journal (Refereed) Published
Abstract [en]

Stem cell research and the prospect of stem cell based therapies depend critically on the identification of specific markers that can be used for the identification and selection of stem and progenitor cells. Nestin is expressed in neuronal progenitor cells and has also been suggested to mark multipotent pancreatic stem cells. We show here that, throughout pancreatic development, markers of pancreatic progenitor cells and differentiated pancreatic cells are expressed in E-cadherin-positive epithelial cells that do not express nestin. The data presented demonstrate that nestin is expressed in mesenchymal and not epithelial cells of the developing mouse pancreas.

Place, publisher, year, edition, pages
Ireland: Elsevier Science , 2002. Vol. 113, no 2, 189-192 p.
Keyword [en]
neuronal progenitors; nestin; pancreatic stem cells; embryonic stem cells; embryonic pancreas; epithelium; mesenchyme; insulin; E-cadherin; IPF1/PDX1; ngn3; Isl1; p48
National Category
Developmental Biology
Research subject
Developmental Neurosciences
Identifiers
URN: urn:nbn:se:umu:diva-25796OAI: oai:DiVA.org:umu-25796DiVA: diva2:233894
Projects
In vivo and in vitro approaches to induce beta cells from stem and progenitor cells
Available from: 2009-09-03 Created: 2009-09-03 Last updated: 2009-09-09Bibliographically approved
In thesis
1. In vivo and in vitro approaches to induce beta cells from stem and progenitor cells
Open this publication in new window or tab >>In vivo and in vitro approaches to induce beta cells from stem and progenitor cells
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Diabetes or diabetes mellitus which is the correct medical term is a medical condition were the affected person lack the ability to regulate his or her blood glucose levels. This inability is directly due to the fact that the insulin producing cells, residing in the pancreas, can’t meet the body’s demand for insulin. It is estimated that close to 200 million people are suffering from diabetes today and this number is predicted to double within 20 years. Of the approximately 200 million people suffering from diabetes today approximately 20 million are in dependent on daily injections of insulin. Being dependent on exogenous insulin is not only an inconvenience it also increase the risk for several medical complications such as stroke, heart disorders, kidney failure, retinopathy, atherosclerosis and impaired wound healing. The major risk factor for all these complications is long periods of high blood sugar levels that is damaging to thin blood vessels and nerves.  Even in the best of situations the blood sugar levels of a diabetic with need for daily insulin injections can never be as well controlled as in a healthy individual.

Increased understanding in the developmental processes behind the formation of the pancreas, and more specifically the insulin producing β-cells could result in new treatments for diabetics. By imitating the in vivo conditions generating pancreatic development scientist are now able to induce embryonic stem cells to differentiate into pancreatic progenitors as well as insulin producing β-cells in vitro. These in vitro generated pancreatic cells might in the future serve as a donor source for transplantations, thereby restoring the insulin producing capability of diabetic patients. An alternative approach to restore insulin production in diabetics is to influence cells in the pancreas to generate more insulin producing cells. To successfully achieve this, what cell types have the capacity to generate β-cells needs to be appreciated.

In this thesis papers concerning in vitro differentiating of embryonic stem cells towards a pancreatic fate as well as in vivo studies in basic pancreas development are presented and discussed.

Publisher
92 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1287
Keyword
pancreas, stem cell, progenitor, beta cell, in vitro
National Category
Medical Genetics
Research subject
Molecular Medicine
Identifiers
urn:nbn:se:umu:diva-25813 (URN)978-91-7268-846-3 (ISBN)
Distributor:
Umeå centrum för molekylär medicin (UCMM), 90185, Umeå
Public defence
2009-09-25, Major Groove, Byggnad 6L, Umeå, 13:00 (English)
Opponent
Supervisors
Available from: 2009-09-09 Created: 2009-09-03 Last updated: 2010-01-18Bibliographically approved

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