Imbalance in tetrapyrrole metabolites caused by deletion of scp genes in the cyanobacterium Synechocystis sp. PCC 6803 results in dramatic cellular effects
(English)Manuscript (preprint) (Other academic)
Light-harvsting like (Lil) proteins contain a chlorophyll-binding domain similar to the chlorophyll a/b binding antenna proteins of plants. The five small Cab-like proteins (SCPs) of the cyanobacterium Synechocystis sp. PCC 6803 belong to the Lil family. They have been shown to stabilize chlorophyll and to play a role in the tetrapyrrole biosynthesis pathway (Xu, H., Vavilin, D., Funk, C. and Vermaas, W. (2004) J. Biol. Chem. 279, 27971-27979). Here we show that deletion of the five scp genes in a PSI-less mutant results in a decrease of Photosystem II amount in the thylakoid membrane. This reduction of Photosystem II leads to increased cell volume, disorganized thylakoid membranes in the cell, and metabolic imbalance. We conclude that the lack of SCPs alters the assembly/repair of Photosystem II, causing an imbalance in the energetic level of the mutant cells.
Photosynthesis, Photosystem II, chlorophyll-binding proteins, tetrapyrrole biosynthesis, photodamage, non-photochemical quenching, SCPs, LHCII
Biochemistry and Molecular Biology Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:umu:diva-25911OAI: oai:DiVA.org:umu-25911DiVA: diva2:234785
ProjectsThe Small Cab-like Proteins in the cyanobacterium Synechocystis sp. PCC 6803