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Imbalance in tetrapyrrole metabolites caused by deletion of scp genes in the cyanobacterium Synechocystis sp. PCC 6803 results in dramatic cellular effects
Umeå University, Faculty of Science and Technology, Chemistry. (Christiane Funk)
Umeå University, Faculty of Science and Technology, Chemistry. (Christiane Funk)
2Unité de Recherche Associée 2096, Centre National de la Recherche Scientifique, Service de Bioénergétique, 91191 Gif sur Yvette, France. (Diana Kirilovsky)
Umeå University, Faculty of Science and Technology, Chemistry. (Christiane Funk)
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Light-harvsting like (Lil) proteins contain a chlorophyll-binding domain similar to the chlorophyll a/b binding antenna proteins of plants. The five small Cab-like proteins (SCPs) of the cyanobacterium Synechocystis sp. PCC 6803 belong to the Lil family. They have been shown to stabilize chlorophyll and to play a role in the tetrapyrrole biosynthesis pathway (Xu, H., Vavilin, D., Funk, C. and Vermaas, W. (2004) J. Biol. Chem. 279, 27971-27979). Here we show that deletion of the five scp genes in a PSI-less mutant results in a decrease of Photosystem II amount in the thylakoid membrane. This reduction of Photosystem II leads to increased cell volume, disorganized thylakoid membranes in the cell, and metabolic imbalance. We conclude that the lack of SCPs alters the assembly/repair of Photosystem II, causing an imbalance in the energetic level of the mutant cells.

Keyword [en]
Photosynthesis, Photosystem II, chlorophyll-binding proteins, tetrapyrrole biosynthesis, photodamage, non-photochemical quenching, SCPs, LHCII
National Category
Biochemistry and Molecular Biology Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:umu:diva-25911OAI: oai:DiVA.org:umu-25911DiVA: diva2:234785
Projects
The Small Cab-like Proteins in the cyanobacterium Synechocystis sp. PCC 6803
Available from: 2009-09-10 Created: 2009-09-10 Last updated: 2010-01-14
In thesis
1. The Small Cab-like Proteins in the cyanobacterium Synechocystis sp. PCC 6803
Open this publication in new window or tab >>The Small Cab-like Proteins in the cyanobacterium Synechocystis sp. PCC 6803
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The Small Cab-like Proteins (SCPs) in the cyanobacterium Synechocystis sp. PCC 6803 accumulate in cells grown under different stress conditions. Genes coding for SCPs have been found in all sequenced organisms performing oxygenic photosynthesis and even in the genomes of cyanophages. Deletion of multiple scp genes in Synechocystis resulted in mutants with severely impaired growth and altered pigment content. These findings indicate the importance of SCPs in photosynthesis; however, their specific function is not well understood. SCPs share a chlorophyll-binding motif with the plant light harvesting complex, suggesting that they bind chlorophyll. Here I describe my findings, which unambiguously show that SCPs are able to bind chlorophyll in vitro. Although they affect both the stoichiometric ratio of Photosystem I to II and chlorophyll stability, they do not seem to be directly involved in non-photochemical quenching. I was able to reveal the location of the SCPs within the cyanobacterial cell: in stressed cells they attach to Photosystem II in the thylakoid membrane. Furthermore, I revealed the presence of another light-harvesting like (Lil)/SCP protein in Synechocystis sp. PCC 6803. The gene, slr1544, codifying for this newly characterised LilA protein, co-transcribes together with scpD and also appears to bind to Photosystem II during stress.

Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2009. 49 p.
Keyword
Photosynthesis, Photosystem II, chlorophyll-binding proteins, tetrapyrrole biosynthesis, photodamage, non-photochemical quenching, SCPs, LHCII
National Category
Biochemistry and Molecular Biology Botany
Research subject
Biochemistry
Identifiers
urn:nbn:se:umu:diva-25886 (URN)978-91-7264-849-4 (ISBN)
Distributor:
Kemi, 90187, Umeå
Public defence
2009-10-02, KB3B9, KBChuset, Umeå Universitet, Umeå, 13:00 (English)
Opponent
Supervisors
Available from: 2009-09-11 Created: 2009-09-09 Last updated: 2009-09-11Bibliographically approved

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