umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
CLE/RLK regulated vascular signalling pathways in plants
Umeå University, Faculty of Science and Technology, Plant Physiology.
2009 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Entire postembryonic production of plant tissues are maintained by meristems. These specialized structures provide a pool of undifferentiated stem cells and a limited population of proliferating cells which are destined for differentiation in order to generate a variety of tissues in the plant body. For the forest trees, a large part of the biomass is produced by a secondary meristem called vascular cambium. Vascular cambium forms a continuous cylinder of meristematic cells in the stem, producing both secondary phloem and secondary xylem or wood. Maintenance and differentiation of meristems are much conserved and strictly regulated for the production of correct tissues and organs. Receptor-like kinases (RLKs) are characterized by the presence of a signal sequence, a putative amino-terminal extracellular domain connected to a carboxyl-terminal intracellular kinase domain with a trans-membrane domain. They control a wide-range of physiological processes, including development, disease resistance, hormone perception, and self-incompatibility. Leucine-rich repeat receptor-like kinases (LRR-RLKs) represent the largest group of RLKs in the Arabidopsis thaliana genome, with more than 200 members.Several LRR-RLKs and their putative ligands CLAVATA3 (CLV3)/ Endosperm Surrounding Region (ESR)-related (CLE) peptides have been found to be involved in the regulation of vascular development. In the current study, the main aim was to study the tissue-specific expression patterns of LRR-RLK genes in A. thaliana by generating promoter::GUS transcriptional fusions. The results confirmed that these genes are expressed in the vasculature of the plants. Moreover, Populus orthologs of the CLE genes were detected by bioinformatic tools as putative ligands of LRR-RLKs and an extensive quantitative Real-Time Reverse Transcriptase PCR (qRT-PCR) analysis was performed to test for significant changes in transcript levels across different tissue types. As a result, a collection of potential candidate genes for vascular development were identified.

Place, publisher, year, edition, pages
2009. , 46 p.
Identifiers
URN: urn:nbn:se:umu:diva-26276OAI: oai:DiVA.org:umu-26276DiVA: diva2:241380
Presentation
KBF30, UPSC, Umeå universitet, Umeå (English)
Uppsok

Supervisors
Examiners
Available from: 2009-10-02 Created: 2009-10-02 Last updated: 2009-10-02Bibliographically approved

Open Access in DiVA

fulltext(5424 kB)645 downloads
File information
File name FULLTEXT01.pdfFile size 5424 kBChecksum SHA-512
eb4b9e5b6107d80d0033e017cdf78395898d79a2455bb92b6d4cba797fceb28df74775b7608e954132f92215b4f1ec6b5a66281c2daf64bf49d1be2ea965c06a
Type fulltextMimetype application/pdf

By organisation
Plant Physiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 645 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 367 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf