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Gene expression in peripheral blood leukocytes in monozygotic twins discordant for chronic fatigue: no evidence of a biomarker
Dept Genetics and dept Biostatistics, University of North Carolina.
Dept Medical Epidemiology and Biostatistics, Karolinska Institutet.
Dept Biosciences and Nutrition, Karolinska Institutet.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
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2009 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 4, no 6, e5805- p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Chronic fatiguing illness remains a poorly understood syndrome of unknown pathogenesis. We attempted to identify biomarkers for chronic fatiguing illness using microarrays to query the transcriptome in peripheral blood leukocytes.

METHODS: Cases were 44 individuals who were clinically evaluated and found to meet standard international criteria for chronic fatigue syndrome or idiopathic chronic fatigue, and controls were their monozygotic co-twins who were clinically evaluated and never had even one month of impairing fatigue. Biological sampling conditions were standardized and RNA stabilizing media were used. These methodological features provide rigorous control for bias resulting from case-control mismatched ancestry and experimental error. Individual gene expression profiles were assessed using Affymetrix Human Genome U133 Plus 2.0 arrays.

FINDINGS: There were no significant differences in gene expression for any transcript.

CONCLUSIONS: Contrary to our expectations, we were unable to identify a biomarker for chronic fatiguing illness in the transcriptome of peripheral blood leukocytes suggesting that positive findings in prior studies may have resulted from experimental bias.

Place, publisher, year, edition, pages
2009. Vol. 4, no 6, e5805- p.
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:umu:diva-27082DOI: 10.1371/journal.pone.0005805PubMedID: 19503787OAI: oai:DiVA.org:umu-27082DiVA: diva2:276090
Available from: 2009-11-10 Created: 2009-11-10 Last updated: 2017-12-12Bibliographically approved

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CiteExportLink to record
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