Detection of functional P fimbriae proteins in E. coli outer membrane vesicles
(English)Manuscript (preprint) (Other academic)
Background: Outer membrane vesicles (OMVs) are continuously released from the surface of Gram negative bacteria. The composition of the OMVs is similar to that of the outer membrane and may include periplasmic constituents. Studies have shown that OMVs also may have a role in the delivery of bacterial toxins to the extracellular space. Results: In this study we investigate the association of P fimbriae proteins to OMVs from both genetically manipulated E. coli laboratory strains and a uropathogenic isolate. We found that the OMVs could carry functionally active P fimbriae proteins on their surface and we observed specific attachment of OMVs to beads coated with the P fimbrial galabiose receptor. Conclusions: Our results suggest that OMVs can carry functionally active fimbrial proteins on their surface and that they may have a role in specific delivery of bacterial components to target cells.
Eshcerichia coli, outer membrane vesicles, OMVs, P fimbriae, adhesion
Microbiology in the medical area
Research subject Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-27737OAI: oai:DiVA.org:umu-27737DiVA: diva2:277440