T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components.
2002 (English)In: European Journal of Immunology, ISSN 0014-2980, Vol. 32, no 4, 1069-1079 p.Article in journal (Refereed) Published
The mechanisms controlling the formation of pseudopodia and other active cell edges in T lymphocytes are not understood. We show here that T lymphocytes express thrombospondin-1 (TSP-1). TSP-1 in T lymphocytes has a high turnover as shown by the fact that brefeldin and monensin rapidly increase while cycloheximide tend to decrease the cellular TSP-1 content. T cell TSP-1 is preferentially stored intracellularly and shows variable cell surface expression. T lymphocyte adhesion to fibronectin and collagen type IV induces TSP-1 expression on the cell surface via a brefeldin sensitive mechanism. A monoclonal antibody to TSP-1 inhibits the flattening and pseudopodia formation of the adherent T cells. Furthermore, the same antibody to TSP-1 also exerts an inhibitory effect on T cell migration in the absence of exogenous TSP-1. These results indicate that endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes.
Place, publisher, year, edition, pages
2002. Vol. 32, no 4, 1069-1079 p.
IdentifiersURN: urn:nbn:se:umu:diva-27748DOI: 10.1002/1521-4141(200204)32:4<1069::AID-IMMU1069>3.0.CO;2-EPubMedID: 11920574OAI: oai:DiVA.org:umu-27748DiVA: diva2:277464