Detection of polyunsaturated omega-6 fatty acid in human malignant prostate tissue by 1D and 2D high-resolution magic angle spinning NMR spectroscopy
2009 (English)In: Magnetic Resonance Materials in Physics, Biology and Medicine, ISSN 0968-5243, E-ISSN 1352-8661, Vol. 22, no 6, 327-331 p.Article in journal (Refereed) Published
OBJECT: Polyunsaturated omega-6 fatty acids (PUFAs) have been shown to promote prostate cancer. Here, we describe the use of HRMAS NMR spectroscopy to detect omega-6 PUFA species in prostate tissues. MATERIALS AND METHODS: Samples originating from non-malignant (n = 54) and malignant (n = 27) prostate tissues (from 27 prostatectomized men) were studied by 1D (1)H, 2D (1)H-(1)H and (1)H-(13)C HRMAS NMR spectroscopy followed by histopathological characterization. RESULTS: HRMAS NMR proved to be a powerful, non-destructive method to identify and characterize PUFAs. The omega-6 PUFA was found in 15% of examined human prostate tumors. CONCLUSION: It is possible to detect PUFAs in prostate tissues using our NMR-based spectroscopic approach.
Place, publisher, year, edition, pages
Berlin: Springer , 2009. Vol. 22, no 6, 327-331 p.
HRMAS NMR, Omega-6 & omega-3 fatty acids, PUFA, Prostate cancer, PCa
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-29882DOI: 10.1007/s10334-009-0187-xPubMedID: 19921294OAI: oai:DiVA.org:umu-29882DiVA: diva2:278422