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Detection of polyunsaturated omega-6 fatty acid in human malignant prostate tissue by 1D and 2D high-resolution magic angle spinning NMR spectroscopy
Umeå University, Faculty of Medicine, Department of Radiation Sciences, Diagnostic Radiology.
Umeå University, Faculty of Medicine, Department of Radiation Sciences, Radiation Physics.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Urology and Andrology.
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2009 (English)In: Magnetic Resonance Materials in Physics, Biology and Medicine, ISSN 0968-5243, E-ISSN 1352-8661, Vol. 22, no 6, 327-331 p.Article in journal (Refereed) Published
Abstract [en]

OBJECT: Polyunsaturated omega-6 fatty acids (PUFAs) have been shown to promote prostate cancer. Here, we describe the use of HRMAS NMR spectroscopy to detect omega-6 PUFA species in prostate tissues. MATERIALS AND METHODS: Samples originating from non-malignant (n = 54) and malignant (n = 27) prostate tissues (from 27 prostatectomized men) were studied by 1D (1)H, 2D (1)H-(1)H and (1)H-(13)C HRMAS NMR spectroscopy followed by histopathological characterization. RESULTS: HRMAS NMR proved to be a powerful, non-destructive method to identify and characterize PUFAs. The omega-6 PUFA was found in 15% of examined human prostate tumors. CONCLUSION: It is possible to detect PUFAs in prostate tissues using our NMR-based spectroscopic approach.

Place, publisher, year, edition, pages
Berlin: Springer , 2009. Vol. 22, no 6, 327-331 p.
Keyword [en]
HRMAS NMR, Omega-6 & omega-3 fatty acids, PUFA, Prostate cancer, PCa
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-29882DOI: 10.1007/s10334-009-0187-xPubMedID: 19921294OAI: diva2:278422
Available from: 2009-11-26 Created: 2009-11-26 Last updated: 2011-08-24Bibliographically approved
In thesis
1. Prostate Cancer Diagnosis: experimental and Clinical Studies With HRMAS NMR Spectroscopy
Open this publication in new window or tab >>Prostate Cancer Diagnosis: experimental and Clinical Studies With HRMAS NMR Spectroscopy
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

A few abnormal cells found in a small piece of prostate tissue are most consequential for a man’s future.

The prevalence of prostate cancer (PCa) is increasing globally. The main instigating factor for this cancer is not yet known, but it appears to be the consequence of many variables such as an increasingly older population, more frequent PSA-testing, and factors involving lifestyle.

Prostate cancer screening, as an equivalent for breast cancer screening, has been suggested but unfortunately there are no accurate diagnostic tools available for this type of screening. The reason for this is simply that the prostate is one of the most difficult organs to diagnose and, consequently, PCa screening would generate far too many false-positive and false-negative results.  The prostate is not easily accessible as it is deeply-seated in the male pelvic area, wrapped around the urethra and surrounded by sensitive vital organs.  Furthermore, PCa is frequently multi-focal, and the cancer cells have a tendency of assimilating among normal cells and, thus, do not always form solid lumps.  Therefore, prostate tumors are often not felt by digital rectal examination (DRE) or identified by imaging.  The PSA-test is not reliable as it is more prostate-specific than cancer-specific.  Due to increasing prostate awareness, more early-stage and locally confined PCa are being detected. This is saving lives, although there is a high risk of over treatment and unnecessary side-effects.  The increased detection of PCa requires sophisticated diagnostic methods and highly skilled clinicians who can discern between indolent and aggressive cancers.  The current “gold-standard” for PCa diagnosis is biopsy grading by pathologists using the Gleason score system, which is a difficult task.  Therefore, innovative methods to improve the precision of prostate diagnosis, by increased biopsy sensitivity and tumor localization, are of essence.

In light of these difficulties, the metabolomic approach using 1D and 2D high-resolution magic angle spinning (HRMAS) NMR spectroscopy combined with histopathology on intact prostatectomy specimens was evaluated in this research project.  The non-destructive nature of HRMAS NMR enables spectroscopic analysis of intact tissue samples with consecutive histological examinations under light microscope. Metabolomics aids in the unraveling and the discovery of organ-specific endogenous metabolites that have the potential to be reliable indicators of organ function and viability, extrinsic and intrinsic perturbations, as well as valuable markers for treatment response. The results may, therefore, be applied clinically to characterize an organ by utilizing biomarkers that have the capacity to distinguish between disease and health.

The aim was to characterize the human and the rat prostate in terms of its intermediary metabolism, which I show here to differ between species and anatomical regions.  Furthermore, the aim is to seek the verification of HRMAS NMR derived metabolites which are known to be a part of the prostate metabolome such as, citrate, choline, and the polyamines which were performed, but also the identification of metabolites not previously identified as part of the local prostate metabolism, such as Omega-6, which was detected in tumors.  The extended aim was to elucidate novel bio-markers with clinical potential. In this study, the common phyto-nutrient, inositol, which appears to possess protective properties, was identified as being a potentially important PCa bio-marker for the distinction between the more indolent Gleason score 6 and the more aggressive Gleason score 7 in non-malignant prostate tissues with tumors elsewhere in the organ. Further studies in this area of PCa research are therefore warranted.

Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2011. 80 p.
Umeå University medical dissertations, ISSN 0346-6612 ; 1406
Prostate cancer, PCa, HRMAS NMR, MRSI, metabolomics, Gleason score, citrate, inositol, choline, Krebs cycle, PUFA, omega-6, omega-3
National Category
Radiology, Nuclear Medicine and Medical Imaging
Research subject
urn:nbn:se:umu:diva-43651 (URN)978-91-7459-154-5 (ISBN)
Public defence
2011-05-27, Sal 260, byggnad 3A, plan 2, Norrlands universitetssjukhus, Umeå, 13:00 (Swedish)
Available from: 2011-05-06 Created: 2011-05-05 Last updated: 2011-05-06Bibliographically approved

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Stenman, KatarinaHauksson, JónGröbner, GerhardStattin, PärBergh, AndersRiklund, Katrine
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