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Translesion synthesis of abasic sites by yeast DNA polymerase epsilon
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
2009 (English)In: The Journal of biological chemistry, ISSN 1083-351X, Vol. 284, no 46, 31555-31563 p.Article in journal (Refereed) Published
Abstract [en]

Studies of replicative DNA polymerases have led to the generalization that abasic sites are strong blocks to DNA replication. Here we show that yeast replicative DNA polymerase epsilon bypasses a model abasic site with comparable efficiency to Pol eta and Dpo4, two translesion polymerases. DNA polymerase epsilon also exhibited high bypass efficiency with a natural abasic site on the template. Translesion synthesis primarily resulted in deletions. In cases where only a single nucleotide was inserted, dATP was the preferred nucleotide opposite the natural abasic site. In contrast to translesion polymerases, DNA polymerase epsilon with 3'-5' proofreading exonuclease activity bypasses only the model abasic site during processive synthesis and cannot reinitiate DNA synthesis. This characteristic may allow other pathways to rescue leading strand synthesis when stalled at an abasic site.

Place, publisher, year, edition, pages
2009. Vol. 284, no 46, 31555-31563 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-30187DOI: 10.1074/jbc.M109.043927PubMedID: 19776424OAI: oai:DiVA.org:umu-30187DiVA: diva2:280464
Available from: 2009-12-10 Created: 2009-12-10 Last updated: 2012-08-15Bibliographically approved

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