Role of LPS in vesicle mediated export of Vibrio cholerae PrtV protease
(English)Manuscript (preprint) (Other academic)
Gram negative bacteria produce outer membrane vesicles (OMVs) during normal bacterial growth. Recent studies have shown that OMVs can transport biologically active toxins and enzymes to the environment and into the host. We have initiated analysis of OMV associated proteins in V. cholerae. In this study, V. cholerae wild type strain P27459 and the O-antigen ligase mutant waaL, which lacks the O-antigen of the LPS were analyzed for the OMV associated release of the secreted protease PrtV. OMVs from the waaL mutant showed a higher amount of associated secreted PrtV protein than the OMVs from wild type V. cholerae indicating that LPS might be involved in vesicle association of the PrtV protein. We showed that the OMV associated PrtV protein is biologically active.
Cell and Molecular Biology
Research subject Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-30256OAI: oai:DiVA.org:umu-30256DiVA: diva2:281249