PCR detection of a hantavirus and Rift Valley fever virus using dried whole blood spotted on filter strips
(English)Manuscript (preprint) (Other academic)
Viral hemorrhagic fevers are serious and emerging infections among humans and animals worldwide. Presently, blood or serum samples are the main source for diagnostics. However, transportation of such samples from remote areas may be complicated and expensive. Previously, filter strips soaked with blood have been used for detection of antibodies for diagnostics and epidemiological studies of several infectious diseases.
In this study we evaluate if a similar approach could be applied for detection of viral RNA of Rift Valley Fever virus or Hantavirus (Puumala).
We have used whole blood spiked with known amounts of viruses. In addition, clinical samples from patients with acute hemorrhagic fever with renal syndrome have been analysed. The samples were collected on filter strips and dried before RNA was extracted at different time-points. For Puumala, the sensitivity was acceptable, although the absolute levels of viral RNA were found to be considerable lower when using filter strips. The viral RNA could be detected and analysed after 2-3 weeks storage of the dried filter strips. In contrast, for RVFV, no or very low copy numbers of viral RNA were detected. Still, RVFV filter strips contained viable virus particles up to 48 h of storage.
In conclusion, the use of dried whole blood samples spotted on filter strips for the detection of viral RNA seems to be a reliable and simple procedure for Hantaviruses. This procedure could be useful in field studies, especially in remote areas or low-income countries where transportation and storage of biological samples might be complicated. However, the result for RVFV was unexpected and further studies are needed to improve this technique.
Rift Valley fever, hantavirus, filter strips
Research subject Medical Virology
IdentifiersURN: urn:nbn:se:umu:diva-30673OAI: oai:DiVA.org:umu-30673DiVA: diva2:285526
Ej publicerat. Not submitted.2010-01-122010-01-122011-10-14Bibliographically approved