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Direct optical detection of aptamer conformational changes induced by target molecules
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2009 (English)In: Analytical chemistry, ISSN 1520-6882, Vol. 81, no 24, 10002-6 p.Article in journal (Refereed) Published
Abstract [en]

Aptamers are single-stranded DNA/RNA oligomers that fold into three-dimensional conformations in the presence of specific molecular targets. Surface-enhanced Raman spectroscopy (SERS) of thiol-bound DNA aptamer self-assembled monolayers on Au nanoshell surfaces provides a direct, label-free detection method for the interaction of DNA aptamers with target molecules. A spectral cross-correlation function, Gamma, is shown to be a useful metric to quantify complex changes in the SERS spectra resulting from conformational changes in the aptamer induced by target analytes. While the pristine, unexposed anti-PDGF (PDGF = platelet-derived growth factor) aptamer yields highly reproducible spectra with Gamma = 0.91 +/- 0.01, following incubation with PDGF, the reproducibility of the SERS spectra is dramatically reduced, yielding Gamma = 0.67 +/- 0.02. This approach also allows us to discriminate the response of a cocaine aptamer to its target from its weaker response to nonspecific analyte molecules.

Place, publisher, year, edition, pages
ACS Publications , 2009. Vol. 81, no 24, 10002-6 p.
Identifiers
URN: urn:nbn:se:umu:diva-30677DOI: 10.1021/ac901849kPubMedID: 19928834OAI: oai:DiVA.org:umu-30677DiVA: diva2:285542
Available from: 2010-01-12 Created: 2010-01-12 Last updated: 2010-01-12Bibliographically approved

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