umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effects of some modulators on purine nucleoside phosphorylase activity in myocardial tissue.
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Anaesthesiology.
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Anaesthesiology.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Medicine.
2009 (English)In: Scand J Clin Lab InvestArticle in journal (Refereed) Published
Abstract [en]

Abstract Purine nucleoside phosphorylase (PNP) in mammalian tissue is an enzyme responsible for formation of purine bases in DNA. It is also believed that PNP is crucial under energy-deprived conditions for the cell to metabolise adenosine during ATP degradation. This work describes a new method for determination of PNP activity in myocardial tissue using a commercially available substrate, 2-amino-6-mercapto-7-methylpurine riboside (MESG). The method involves the photometric assessment of the reaction between PNP (extracted from myocardial tissue) and MESG. Quantification as well as temperature- and pH-dependency for myocardial PNP activity is described. Also, the effect of some modulators has been studied. We have established the presence of PNP activity in pig myocardial tissue. Further, the results indicate a pH tolerance under slightly acid conditions and a calcium ion dependence of the enzyme.

Place, publisher, year, edition, pages
2009.
Identifiers
URN: urn:nbn:se:umu:diva-31400ISBN: 1502-7686 OAI: oai:DiVA.org:umu-31400DiVA: diva2:292951
Available from: 2010-02-10 Created: 2010-02-10 Last updated: 2010-06-03

Open Access in DiVA

fulltext(219 kB)288 downloads
File information
File name FULLTEXT01.pdfFile size 219 kBChecksum SHA-512
c138c8076442cd9b354ea667355bffb9a3b3214415c7f93fb78f5440e5dfee4a26f091964f338e2340f92402c3352c31c7698ab9d034d2638bf211fa4e99f395
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Åberg, AnnaMajaHaney, MichaelWaldenström, Anders
By organisation
AnaesthesiologyMedicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 288 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 98 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf