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Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
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2010 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 29, no 3, 546-58 p.Article in journal (Refereed) Published
Abstract [en]

Cytokinesis represents the final stage of eukaryotic cell division during which the cytoplasm becomes partitioned between daughter cells. The process differs to some extent between animal and plant cells, but proteins of the syntaxin family mediate membrane fusion in the plane of cell division in diverse organisms. How syntaxin localization is kept in check remains elusive. Here, we report that localization of the Arabidopsis KNOLLE syntaxin in the plane of cell division is maintained by sterol-dependent endocytosis involving a clathrin- and DYNAMIN-RELATED PROTEIN1A-dependent mechanism. On genetic or pharmacological interference with endocytosis, KNOLLE mis-localizes to lateral plasma membranes after cell-plate fusion. Fluorescence-loss-in-photo-bleaching and fluorescence-recovery-after-photo-bleaching experiments reveal lateral diffusion of GFP-KNOLLE from the plane of division to lateral membranes. In an endocytosis-defective sterol biosynthesis mutant displaying lateral KNOLLE diffusion, KNOLLE secretory trafficking remains unaffected. Thus, restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis.

Place, publisher, year, edition, pages
2010. Vol. 29, no 3, 546-58 p.
National Category
Plant Biotechnology
Research subject
Physiological Botany
Identifiers
URN: urn:nbn:se:umu:diva-31866DOI: 10.1038/emboj.2009.363ISI: 000274233400004PubMedID: 19959995OAI: oai:DiVA.org:umu-31866DiVA: diva2:297959
Available from: 2010-02-19 Created: 2010-02-19 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Dissecting sterol function during clathrin-dependent endocytosis and cytokinesis in Arabidopsis thaliana
Open this publication in new window or tab >>Dissecting sterol function during clathrin-dependent endocytosis and cytokinesis in Arabidopsis thaliana
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sterols are lipid components of eukaryotic membranes. Alterations of membrane sterol composition perturb the execution of cell division, which in diverse eukaryotes can have severe consequences for development of the organism. Partitioning of the cytoplasm during cell division occurs at the final stage of cell division named cytokinesis. In somatic plant cells, cytokinesis is initiated by fusion of membrane vesicles in the plane of cell division resulting in a transient compartment termed the cell plate. Cell plate maturation relies on temporal and spatial orchestration of membrane fusion and endocytosis. Impaired vesicle fusion or defects in endocytosis result in cytokinetic defects.

In Arabidopsis thaliana, the KNOLLE and DYNAMIN-RELATED PROTEIN 1A (DRP1A) contribute to cytokinesis. KNOLLE mediates fusion of vesicles at the plane of cell division while DRP1A appears to be involved in cell plate maturation through its role in clathrin-mediated endocytosis.

This thesis shows that KNOLLE is specifically restricted to the cell division plane through sterol-dependent endocytosis that involves a clathrin- and DRP1A-mediated mechanism. Sterols affect internalization of KNOLLE through their role in lateral membrane organization by keeping diffusion of KNOLLE to lateral membranes in check via its endocytic removal. It is shown that the cell plate represents a high-lipid-order membrane domain that depends on the correct composition and the right concentration of sterols. Accumulation of DRP1A at the cell plate requires correct sterol concentration and composition similar to high-lipid order. Conversely, high-lipid-order at the cell plate relies on DRP1A activity suggesting a feedback between DRP1A function and lipid order establishment. Finally, it is shown that sterols are also present at the tonoplast of dividing and elongated root cells.

Taken together, the results reveal that formation of the cell plate in Arabidopsis thaliana depends on an intricate interplay between cytokinetic vesicle fusion, sterol-dependent lateral membrane and high-lipid-order domain organization as well as endocytic machinery function.

Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2013. 64 p.
Keyword
Arabidopsis, membrane, sterols, cytokinesis, KNOLLE, endocytosis, clathrin, DRP1A
National Category
Natural Sciences
Research subject
Developmental Biology; Molecular Biology; Molecular Cellbiology
Identifiers
urn:nbn:se:umu:diva-80030 (URN)978-91-7459-721-9 (ISBN)
Public defence
2013-10-04, KBC-huset, KB3A9, Umeå Universitet, Umeå, 10:00 (English)
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Available from: 2013-09-13 Created: 2013-09-09 Last updated: 2015-05-07Bibliographically approved

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Boutté, YohannFrescatada-Rosa, MárciaMen, ShuzhenGustavsson, AnnaJohansson, LenoreGrebe, Markus
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