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Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
2002 (English)In: RNA: A publication of the RNA Society, ISSN 1355-8382, E-ISSN 1469-9001, Vol. 8, no 3, 324-335 p.Article in journal (Refereed) Published
Abstract [en]

A 5-methyluridine (m(5)U) residue at position 54 is a conserved feature of bacterial and eukaryotic tRNAs. The methylation of U54 is catalyzed by the tRNA(m5U54)methyltransferase, which in Saccharomyces cerevisiae is encoded by the nonessential TRM2 gene. In this study, we identified four different strains with mutant forms of tRNA(Ser)CGA. The absence of the TRM2 gene in these strains decreased the stability of tRNA(Ser)CGA and induced lethality. Two alleles of TRM2 encoding catalytically inactive tRNA(m5U54)methyltransferases were able to stabilize tRNA(Ser)CGA in one of the mutants, revealing a role for the Trm2 protein per se in tRNA maturation. Other tRNA modification enzymes interacting with tRNA(Ser)CGA in the maturation process, such as Pus4p, Trm1 p, and Trm3p were essential or important for growth of the tRNA(Ser)CGA mutants. Moreover, Lhp1p, a protein binding RNA polymerase III transcripts, was required to stabilize the mutant tRNAs. Based on our results, we suggest that tRNA modification enzymes might have a role in tRNA maturation not necessarily linked to their known catalytic activity.

Place, publisher, year, edition, pages
2002. Vol. 8, no 3, 324-335 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-32189DOI: 10.1017/S1355838202027851PubMedID: 12003492OAI: oai:DiVA.org:umu-32189DiVA: diva2:301443
Available from: 2010-03-03 Created: 2010-03-03 Last updated: 2017-12-12Bibliographically approved

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Johansson, Marcus J OByström, Anders S
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