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A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene.
Umeå University, Faculty of Medicine, Molecular Biology. Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
1991 (English)In: Molecular and Cellular Biology, ISSN 0270-7306, Vol. 11, no 1, 213-217 p.Article in journal (Refereed) Published
Abstract [en]

Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C.

Place, publisher, year, edition, pages
1991. Vol. 11, no 1, 213-217 p.
National Category
Microbiology in the medical area
URN: urn:nbn:se:umu:diva-32175PubMedID: 1986221OAI: diva2:301475
Available from: 2010-03-03 Created: 2010-03-03 Last updated: 2010-03-12Bibliographically approved

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