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A functional analysis of the repeated methionine initiator tRNA genes (IMT) in yeast
Umeå University, Faculty of Medicine, Molecular Biology. Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
1989 (English)In: Molecular General Genetics, ISSN 0026-8925, E-ISSN 1432-1874, Vol. 216, no 2-3, 276-286 p.Article in journal (Refereed) Published
Abstract [en]

Standard laboratory yeast strains have from four to five genes encoding the methionine initiator tRNA (IMT). Strain S288C has four IMT genes with identical coding sequences that are colinear with the RNA sequence of tRNA(IMet). Each of the four IMT genes from strain S288C is located on a different chromosome. A fifth IMT gene with the same coding sequence is present in strain A364A but not in S288C. By making combinations of null alleles in strain S288C, we show that each of the four IMT genes is functional and that tRNA(IMet) is not limiting in yeast strains with three or more intact genes. Strains containing a single IMT2, 3 or 4 gene grow only after amplification of the remaining IMT gene. Strains with only the IMT1 gene intact are viable but grow extremely slow; normal growth is restored by the addition of another IMT gene by transformation, providing a direct test for IMT function.

Place, publisher, year, edition, pages
1989. Vol. 216, no 2-3, 276-286 p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-32174PubMedID: 2664453OAI: oai:DiVA.org:umu-32174DiVA: diva2:301476
Available from: 2010-03-03 Created: 2010-03-03 Last updated: 2017-12-12Bibliographically approved

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