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Chromosomal location and cloning of the gene (trmD) responsible for the synthesis of tRNA (m1G) methyltransferase in Escherichia coli K-12.
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
1982 (English)In: Molecular General Genetics, ISSN 0026-8925, E-ISSN 1432-1874, Vol. 188, no 3, 440-446 p.Article in journal (Refereed) Published
Abstract [en]

The trmD gene, which governs the formation of 1-methyl-guanosine(m1G) in transfer ribonucleic acid (tRNA), has been located by phage P1 transduction at 56 min on the chromosomal map of Escherichia coli. Cotransduction to tyrA at 56 min is 80%. From the Clarke and Carbon collection a ColE1-tyrA+ hybrid plasmid was isolated, which carried the trmD+ gene and was shown to over-produce the tRNA(m1G)methyltransferase. By subcloning restriction enzyme fragments in vitro, the trmD+ gene was located to a 3.4 kb DNA fragment 6.5 kb clockwise from the tyrA+ gene. The mutation trmD1, which renders the tRNA(m1G)methyltransferase temperature-sensitive both in vivo and in vitro could be complemented by trmD+ plasmids. These results suggest that the gene trmD+ is the structural gene for the tRNA(m1G)methyltransferase (EC 2.1.1.3.1).

Place, publisher, year, edition, pages
1982. Vol. 188, no 3, 440-446 p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-32165PubMedID: 6298573OAI: oai:DiVA.org:umu-32165DiVA: diva2:301562
Available from: 2010-03-03 Created: 2010-03-03 Last updated: 2017-12-12Bibliographically approved

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