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Active cytotoxic necrotizing factor 1 associated with outer membrane vesicles from uropathogenic Escherichia coli.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
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2006 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 74, no 4, 2022-2030 p.Article in journal (Refereed) Published
Abstract [en]

Cytotoxic necrotizing factor type 1 (CNF1) is one of the virulence factors produced by uropathogenic Escherichia coli (UPEC). How this toxin is translocated from the bacterial cytoplasm to the surrounding environment is not well understood. Our data suggest that CNF1 may be regarded as a secreted protein, since it could be detected in culture supernatants. Furthermore, we found that CNF1 was tightly associated to outer membrane vesicles, suggesting that such vesicles play a role in the secretion of this protein. Interestingly, vesicle samples containing CNF1 could exert the effects known for this protein on HeLa cell cultures, showing that CNF1 is transported by vesicles in its active form. Taken together, our results strongly suggest that outer membrane vesicles could be a means for the bacteria to deliver CNF1 to the environment and to the infected tissue. In addition, our results indicate that the histone-like nucleoid structuring protein H-NS has a role in the downregulation of CNF1 production and that it affects the outer membrane vesicle release in UPEC strain J96.

Place, publisher, year, edition, pages
2006. Vol. 74, no 4, 2022-2030 p.
Keyword [en]
Bacterial Outer Membrane Proteins/genetics/*metabolism/secretion, Bacterial Toxins/genetics/*metabolism, Biological Transport; Active, Blotting; Western, Cytotoxins/*metabolism/secretion, DNA-Binding Proteins/physiology, Escherichia coli/genetics/*metabolism/*pathogenicity/ultrastructure, Escherichia coli Proteins/genetics/*metabolism/physiology/secretion, Hela Cells, Humans, Mutation, Transport Vesicles/metabolism
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Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-32249DOI: 10.1128/IAI.74.4.2022-2030.2006PubMedID: 16552031OAI: oai:DiVA.org:umu-32249DiVA: diva2:302230
Available from: 2010-03-04 Created: 2010-03-04 Last updated: 2017-12-12Bibliographically approved

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Kouokam, J ClavinWai, Sun NyuntFällman, MariaUhlin, Bernt Eric
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Department of Molecular Biology (Faculty of Medicine)Umeå Centre for Microbial Research (UCMR)
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