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Identification of cell surface determinants in Candida albicans reveals Tsa1p, a protein differentially localized in the cell.
Umeå University, Faculty of Medicine, Molecular Biology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). (Urban)
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2003 (English)In: FEBS Letters, ISSN 0014-5793, Vol. 544, no 1-3, 228-235 p.Article in journal (Refereed) Published
Abstract [en]

To identify cell surface proteins of Candida albicans, the predominant fungal pathogen in humans, we have established an approach using a membrane impermeable biotin derivative in combination with affinity purification. We were able to identify 29 different proteins under two distinct conditions. Among mannoproteins, heat shock proteins and glycolytic enzymes we found thiol-specific antioxidant-like protein 1 (Tsa1p) to be differentially localized depending on the conditions applied. Only in hyphally grown cells Tsa1p was localized to the cell surface whereas in blastospores no surface but mainly nuclear localization was found. This indicates that cell surface expression of at least some proteins is mediated by differential translocation.

Place, publisher, year, edition, pages
2003. Vol. 544, no 1-3, 228-235 p.
Keyword [en]
Candida albicans, Cell surface protein, Surface biotinylation, Affinity chromatography, Thiol-specific antioxidant-like protein
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-32558DOI: 10.1016/S0014-5793(03)00455-1PubMedID: 12782322OAI: diva2:304070
Available from: 2010-03-16 Created: 2010-03-16 Last updated: 2010-04-09Bibliographically approved

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