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NF-kappaB only partially mediates Epstein-Barr virus latent membrane protein 1 activation of B cells.
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine).
1998 (English)In: Journal of General Virology, ISSN 0022-1317, E-ISSN 1465-2099, Vol. 79 ( Pt 9), 2117-25 p.Article in journal (Refereed) Published
Abstract [en]

The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is required for EBV-induced immortalization of human B cells and causes tumorigenic transformation of cell lines. LMP1 expression induces phenotypic changes resembling B cell activation, such as cell size increase and up-regulation of cell surface activation markers. LMP1 contains two domains that activate the transcription factor NF-kappaB, one through interactions with TRAF proteins and the other with the TRADD protein. The purpose of the present study was to investigate the importance of NF-kappaB induction in the up-regulation of the B cell activation markers ICAM-1 and CD71 by LMP1. This study shows that expression of LMP1 activates transcription from p50/p65- and c-Rel-responsive promoters, and that this activity can be completely inhibited by expression of a dominant inhibitory IkappaB mutant. ICAM-1 and CD71 are nevertheless up-regulated by LMP1 in primary B cells and cell lines expressing the dominant IkappaB. Furthermore, LMP1-induced cell size increase of primary B cells was unaffected by IkappaB expression. It was concluded that even when LMP1 is unable to activate NF-kappaB, it is still capable of inducing certain characteristics of activated B cells, strongly suggesting that LMP1 can also activate cells independently of NF-kappaB.

Place, publisher, year, edition, pages
1998. Vol. 79 ( Pt 9), 2117-25 p.
Identifiers
URN: urn:nbn:se:umu:diva-33076PubMedID: 9747720OAI: oai:DiVA.org:umu-33076DiVA: diva2:309933
Available from: 2010-04-09 Created: 2010-04-09 Last updated: 2017-12-12

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