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Gene expression in transfected cells
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2002 (English)In: Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques / [ed] Hans J. Vogel, Totowa, NJ: Humana Press, 2002, Vol. 173, 355-363 p.Chapter in book (Refereed)
Abstract [en]

A general approach to address the biological function of a calcium-binding protein, or another protein, in living cells is to increase or decrease the activity of the protein in the cell and analyze the effects on cell functions. In many cases, it is desirable to determine the effects of overexpressing the protein or a constitutively active or dominantly negative derivative, or to express the protein in a cell that normally lacks it. This is achieved by introducing its gene exogenously. The cDNA for the protein is cloned downstream of an active promoter in a plasmid designed for expression in mammalian cells. This expression plasmid is then transfected into the cell.

Place, publisher, year, edition, pages
Totowa, NJ: Humana Press, 2002. Vol. 173, 355-363 p.
Series
Methods in molecular biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 173
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-33071DOI: 10.1385/1-59259-184-1:355PubMedID: 11859775ISBN: 978-0-89603-689-5 (print)OAI: oai:DiVA.org:umu-33071DiVA: diva2:309938
Available from: 2010-04-09 Created: 2010-04-09 Last updated: 2017-12-13Bibliographically approved

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Grundström, Thomas
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CiteExportLink to record
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