Gene expression in transfected cells.
2002 (English)In: Methods in Molecular Biology, ISSN 1064-3745, Vol. 173, 355-363 p.Article in journal (Refereed) Published
A general approach to address the biological function of a calcium-binding protein, or another protein, in living cells is to increase or decrease the activity of the protein in the cell and analyze the effects on cell functions. In many cases, it is desirable to determine the effects of overexpressing the protein or a constitutively active or dominantly negative derivative, or to express the protein in a cell that normally lacks it. This is achieved by introducing its gene exogenously. The cDNA for the protein is cloned downstream of an active promoter in a plasmid designed for expression in mammalian cells. This expression plasmid is then transfected into the cell.
Place, publisher, year, edition, pages
2002. Vol. 173, 355-363 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-33071DOI: 10.1385/1-59259-184-1:355PubMedID: 11859775OAI: oai:DiVA.org:umu-33071DiVA: diva2:309938