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Population dynamics of a single-stage sulfidogenic bioreactor treating synthetic zinc-containing waste streams
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
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2009 (English)In: Microbial Ecology, ISSN 0095-3628, E-ISSN 1432-184X, Vol. 58, no 3, 529-537 p.Article in journal (Refereed) Published
Abstract [en]

Waste streams from industrial processes such as metal smelting or mining contain high concentrations of sulfate and metals with low pH. Dissimilatory sulfate reduction carried out by sulfate-reducing bacteria (SRB) at low pH can combine sulfate reduction with metal-sulfide precipitation and thus open possibilities for selective metal recovery. This study investigates the microbial diversity and population changes of a single-stage sulfidogenic gas-lift bioreactor treating synthetic zinc-rich waste water at pH 5.5 by denaturing gradient gel electrophoresis of 16S rRNA gene fragments and quantitative polymerase chain reaction. The results indicate the presence of a diverse range of phylogenetic groups with the predominant microbial populations belonging to the Desulfovibrionaceae from delta-Proteobacteria. Desulfovibrio desulfuricans-like populations were the most abundant among the SRB during the three stable phases of varying sulfide and zinc concentrations and increased from 13% to 54% of the total bacterial populations over time. The second largest group was Desulfovibrio marrakechensis-like SRB that increased from 1% to about 10% with decreasing sulfide concentrations. Desulfovibrio aminophilus-like populations were the only SRB to decrease in numbers with decreasing sulfide concentrations. However, their population was <1% of the total bacterial population in the reactor at all analyzed time points. The number of dissimilatory sulfate reductase (DsrA) gene copies per number of SRB cells decreased from 3.5 to 2 DsrA copies when the sulfide concentration was reduced, suggesting that the cells' sulfate-reducing capacity was also lowered. This study has identified the species present in a single-stage sulfidogenic bioreactor treating zinc-rich wastewater at low pH and provides insights into the microbial ecology of this biotechnological process.

Place, publisher, year, edition, pages
2009. Vol. 58, no 3, 529-537 p.
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URN: urn:nbn:se:umu:diva-33097DOI: 10.1007/s00248-009-9509-9PubMedID: 19322604OAI: diva2:310077
Available from: 2010-04-12 Created: 2010-04-12 Last updated: 2012-05-10Bibliographically approved

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Dar, Shabir ADopson, Mark
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Department of Molecular Biology (Faculty of Science and Technology)
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