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Control of Listeria monocytogenes virulence by 5´-untranslated RNA
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Jörgen Johansson)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Jörgen Johansson)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Jörgen Johansson)
2006 (English)In: Trends in Microbiology, ISSN 0966-842X, E-ISSN 1878-4380, Vol. 14, no 7, 294-298 p.Article in journal (Refereed) Published
Abstract [en]

The Gram-positive bacterium Listeria monocytogenes uses a wide range of virulence factors for its pathogenesis. Expression of five of these factors has previously been shown to be subjected to posttranscriptional regulation as a result of their long 5´-untranslated region (5´-UTR). We have investigated the presence of 5´-UTRs among the other known virulence genes and genes that encode putatively virulence-associated surface proteins. Our results strongly suggest that L. monocytogenes controls many of its virulence genes by a mechanism that involves the 5´-UTR. These findings further emphasize the importance of post-transcriptional control for L. monocytogenes virulence.

Place, publisher, year, edition, pages
2006. Vol. 14, no 7, 294-298 p.
Keyword [en]
5´-UTRs, Listeria monocytogenes, virulence genes
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-33106DOI: 10.1016/j.tim.2006.05.001OAI: oai:DiVA.org:umu-33106DiVA: diva2:310105
Available from: 2010-04-12 Created: 2010-04-12 Last updated: 2017-12-12Bibliographically approved
In thesis
1. RNA-mediated virulence gene regulation in the human pathogen Listeria monocytogenes
Open this publication in new window or tab >>RNA-mediated virulence gene regulation in the human pathogen Listeria monocytogenes
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The Gram-positive human pathogen Listeria monocytogenes uses a wide range of virulence factors for its pathogenesis. The majority of its virulence genes are encoded on a 9-kb pathogenicity island and are controlled by the transcriptional activator PrfA. Expression of these genes is maximal at 37°C and minimal at 30°C in a mechanism involving an RNA thermosensor. This thesis brings up different aspects of RNA-mediated regulation, including regulatory RNA structures within coding mRNA controlling expression to 5-untranslated RNA (5´-UTR) that controls downstream genes (cis-acting) as well as small non-coding RNAs (ncRNAs) that bind other target RNA (trans-acting).

We investigated the importance of the coding region of the prfA-mRNA for its expression. Various lengths of prfA-mRNA were fused with reporter genes. Our finding suggested that the first 20 codons of prfA-mRNA were essential for efficient translation in Listeria monocytogenes. Translation of the shorter constructs was shown to be reduced. The expression level showed an inverse correlation with the RNA secondary structure stability in the beginning of the coding region. Riboswitches have previously been known to control expression of their downstream mRNA in a cis-acting manner. A trans-acting S-adenosylmethionine-binding riboswitch termed SreA was identified in Listeria monocytogenes. It was found to control the expression of the virulence regulator PrfA, by binding to the prfA-UTR and thereby affecting its translation. We examined the RNA locus encoding different virulence factors in Listeria monocytogenes. Several of them were preceded by 5´-UTRs of various lengths. We speculate that these 5´-UTRs could control expression of the downstream mRNA, provided they are of sufficient length. These findings prompted us to examine where and when Listeria monocytogenes switches on gene expression. Tiling array was used to compare RNAs isolated from wild-type and mutant bacteria grown at different growth conditions. Antisense RNAs covering parts of or whole open-reading frames as well as 29 new ncRNAs were identified. Several novel riboswitches possibly functioning as upstream terminators were also found.

My thesis work compiles together a variety of novel RNA-mediated gene regulatory entities. A first coordinated transcriptional map of Listeria monocytogenes has been set up. My work has also revealed that the expression of the virulence regulator PrfA is controlled at several levels, indicating the importance of both the 5´-UTR and the coding RNA for regulated expression.

Place, publisher, year, edition, pages
Umeå, Sweden: Print & Media, 2010. 90 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1337
Keyword
Listeria monocytogenes, virulence, ncRNAs, PrfA, 5´-UTRs
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-33096 (URN)0346-6612 (ISBN)
Public defence
2010-05-12, Major Groove, Department of Molecular Biology, Umeå University, 10:00 (English)
Opponent
Supervisors
Available from: 2010-04-20 Created: 2010-04-12 Last updated: 2010-04-20Bibliographically approved

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Loh, EdmundGripenland, JonasJohansson, Jörgen

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