Change search
ReferencesLink to record
Permanent link

Direct link
Non-linear effects of macromolecular crowding on enzymatic activity of multi-copper oxidase
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2010 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, Vol. 1804, no 4, 740-744 p.Article in journal (Refereed) Published
Abstract [en]

Enzymes catalyze biochemical reactions in highly crowded environments where the amount of macromolecules may occupy up to 40% of the volume. Here we report how cell-like conditions tune catalytic parameters for the monomeric multi-copper oxidase, Saccharomyces cerevisiae Fet3p, in vitro. At low amounts of crowding agent, we detect increases in both of K(M) (weaker substrate binding) and k(cat) (improved catalytic efficiency), whereas at higher crowding levels, both parameters were reduced. Presence of crowding agents does not affect Fet3p structural content but increases thermal resistance. The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics as a result of excluded volume effects making the protein less structurally 'strained'.

Place, publisher, year, edition, pages
2010. Vol. 1804, no 4, 740-744 p.
Keyword [en]
Enzyme activity, Macromolecular crowding, Circular dichroism, Thermal stability, Crowding agent
URN: urn:nbn:se:umu:diva-33190DOI: 10.1016/j.bbapap.2009.11.013ISI: 000275516500011PubMedID: 19932772OAI: diva2:310707
Available from: 2010-04-15 Created: 2010-04-15 Last updated: 2010-04-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Pozdnyakova, IrinaWittung-Stafshede, Pernilla
By organisation
Department of Chemistry
In the same journal
Biochimica et Biophysica Acta

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 124 hits
ReferencesLink to record
Permanent link

Direct link