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Pigment epithelium-derived factor stimulates tumor macrophage recruitment and is downregulated by the prostate tumor microenvironment
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Urologi och andrologi.
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2010 (Engelska)Ingår i: Neoplasia, ISSN 1522-8002, E-ISSN 1476-5586, Vol. 12, nr 4, s. 336-345Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis but whether it has additional effects on the tumor microenvironment is largely unexplored. We show that overexpression of PEDF in orthotopic MatLyLu rat prostate tumors increased tumor macrophage recruitment. The fraction of macrophages expressing inducible nitric oxide synthase, a marker of cytotoxic M1 macrophages, was increased, suggesting that PEDF could enhance antitumor immunity. In addition, PEDF overexpression reduced vascular growth both in the tumor and in the surrounding normal tissue, slowed tumor growth, and decreased lymph node metastasis. Contrary, extratumoral lymphangiogenesis was increased. PEDF expression is, for reasons unknown, often decreased or lost during prostate tumor progression. When AT-1 rat prostate tumor cells, expressing high levels of PEDF messenger RNA (mRNA) and protein, were injected into the prostate, PEDF is markedly downregulated, suggesting that factors in the microenvironment suppressed its expression. One such factor could be macrophage-derived tumor necrosis factor alpha (TNFα). A fraction of the accumulating macrophages expressed TNFα, and TNFα treatment downregulated the expression of PEDF protein and mRNA in prostate AT-1 tumor cells in vitro and in the rat ventral prostate in vivo. PEDF apparently has multiple effects in prostate tumors: it suppresses angiogenesis and metastasis, but it also causes macrophage accumulation. Accumulating macrophages may inhibit tumor growth, but they may also suppress PEDF and enhance lymph angiogenesis and, in this way, eventually enhance tumor growth.

Ort, förlag, år, upplaga, sidor
Neoplasia press , 2010. Vol. 12, nr 4, s. 336-345
Nationell ämneskategori
Cancer och onkologi
Identifikatorer
URN: urn:nbn:se:umu:diva-33268DOI: 10.1593/neo.92046ISI: 000277646000005PubMedID: 20360944OAI: oai:DiVA.org:umu-33268DiVA, id: diva2:311154
Tillgänglig från: 2010-04-20 Skapad: 2010-04-20 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Ingår i avhandling
1. Targeting the prostate tumor microenvironment and vasculature: the role of castration, tumor-associated macrophages and pigment epithelium-derived factor
Öppna denna publikation i ny flik eller fönster >>Targeting the prostate tumor microenvironment and vasculature: the role of castration, tumor-associated macrophages and pigment epithelium-derived factor
2009 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Alternativ titel[sv]
Mikromiljö och angiogenes i prostatacancer : effekter av kastration, tumör associerade makrofager och Pigment epithelium-derived factor
Abstract [en]

BACKGROUND: Prostate cancer is the most common cancer among Swedish men. For patients with metastatic prostate cancer the standard therapy is castration, a treatment that initially provides symptomatic relief but unfortunately is not curative. New therapeutic targets for advanced prostate cancer are therefore needed.  Prostate cancers are composed of tumor epithelial cells as well as many non-epithelial cells such as cancer associated fibroblasts, blood vessels and inflammatory cells.  Many components of the tumor microenvironment such as tumor associated macrophages and angiogenesis have been shown to stimulate tumor progression. This thesis aims to explore mechanisms by which the local environment influences prostate tumor growth and how such mechanisms could be targeted for treatment.

MATERIALS AND METHODS: We have used animal models of prostate cancer, in vitro cell culture systems and clinical materials from untreated prostate cancer patients with long follow up. Experiments were evaluated with stereological techniques, immunohistochemistry, western blotting, quantitative real-time PCR, PCR arrays and laser micro dissection.

RESULTS: We found that the presence of a tumor induces adaptive changes in the surrounding non-malignant prostate tissue, and that androgen receptor negative prostate tumor cells respond to castration treatment with temporarily reduced growth when surrounded by normal castration-responsive prostate tissue. Further, we show that macrophages are important for prostate tumor growth and angiogenesis in the tumor and in the surrounding non-malignant tissue. In addition, the angiogenesis inhibitor Pigment epithelium-derived factor (PEDF) was found  to be down-regulated in metastatic rat and human prostate tumors. Over-expression of PEDF inhibited experimental prostate tumor growth, angiogenesis and metastatic growth and stimulated macrophage tumor infiltration and lymphangiogenesis. PEDF was found to be down-regulated by the prostate microenvironment and tumor necrosis factor (TNF) α.

CONCLUSIONS: Our studies indicate that not only the nearby tumor microenvironment but also the surrounding non-malignant prostate tissue are important for prostate tumor growth. Both the tumor and the surrounding non-malignant prostate were characterized by increased angiogenesis and inflammatory cell infiltration. Targeting the surrounding prostate tissue with castration, targeting tumor associated macrophages, or targeting the vasculature directly using inhibitors like PEDF were all shown to repress prostate tumor growth and could prove beneficial for patients with advanced prostate cancer.

Ort, förlag, år, upplaga, sidor
Umeå: Medicinsk biovetenskap, 2009. s. 57
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 1293
Nyckelord
Prostate cancer, angiogenesis, castration therapy, microenvironment, stroma, tumor-associated macrophages, pigment epithelium-derived factor, PEDF, vasculature
Nationell ämneskategori
Cell- och molekylärbiologi
Forskningsämne
patologi
Identifikatorer
urn:nbn:se:umu:diva-30300 (URN)978-91-7264-862-3 (ISBN)
Disputation
2010-01-22, Hörsal Betula, 6M, Norrlands universitetssjukhus, Umeå, 10:00 (Svenska)
Opponent
Handledare
Tillgänglig från: 2009-12-22 Skapad: 2009-12-16 Senast uppdaterad: 2018-01-12Bibliografiskt granskad

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Halin, SofiaHäggström Rudolfsson, StinaWikström, PernillaBergh, Anders

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