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Microtubule-dependent regulation of Rho GTPases during internalisation of Yersinia pseudotuberculosis.
Umeå University, Faculty of Medicine, Molecular Biology.
Umeå University, Faculty of Medicine, Molecular Biology.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
2003 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 533, no 1-3, 35-41 p.Article in journal (Refereed) Published
Abstract [en]

Internalisation of the human pathogen Yersinia pseudotuberculosis via interaction of bacterial invasin with host beta1 integrins depends on the actin cytoskeleton and involves Src family kinases, focal adhesion kinase, p130Crk-associated substrate, proline-rich tyrosine kinase 2, Rac, Arp 2/3 complex and WASP family members. We show here that Rho GTPases are regulated by the microtubule system during bacterial uptake. Interfering with microtubule organisation using nocodazole or paclitaxel suppressed uptake by HeLa cells. The nocodazole effect on microtubule depolymerisation was partially inhibited through overexpression of Rac, Cdc42, RhoG or RhoA and completely prevented by expression of Vav2. This suggests that microtubules influence Rho GTPases during invasin-mediated phagocytosis and in the absence of functional microtubules Vav2 can mimic their effect on one, or more, of the Rho family GTPases. Lastly, overexpression of p50 dynamitin partially inhibited bacterial uptake and this effect was also blocked by co-expression of Vav2, thus further implicating this guanine nucleotide exchange factor in activating Rho GTPases for internalisation during loss of microtubule function.

Place, publisher, year, edition, pages
2003. Vol. 533, no 1-3, 35-41 p.
Keyword [en]
Invasin, Internalization, Microtubule, Rho GTPase, Vav2, Yersinia
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-33366DOI: 10.1016/S0014-5793(02)03745-6PubMedID: 12505155OAI: diva2:311635
Available from: 2010-04-22 Created: 2010-04-22 Last updated: 2010-08-12Bibliographically approved

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McGee, KarenHolmfeldt, PerFällman, Maria
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Molecular BiologyDepartment of Molecular Biology (Faculty of Medicine)Umeå Centre for Microbial Research (UCMR)
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