Pancreatic-duodenal homeobox 1 regulates expression of liver receptor homolog 1 during pancreas development.
2003 (English)In: Molecular and Cellular Biology, ISSN 0270-7306, E-ISSN 1098-5549, Vol. 23, no 19, 6713-6124 p.Article in journal (Refereed) Published
Liver receptor homolog 1 (LRH-1) and pancreatic-duodenal homeobox 1 (PDX-1) are coexpressed in the pancreas during mouse embryonic development. Analysis of the regulatory region of the human LRH-1 gene demonstrated the presence of three functional binding sites for PDX-1. Electrophoretic mobility shift assays and chromatin immunoprecipitation analysis showed that PDX-1 bound to the LRH-1 promoter, both in cultured cells in vitro and during pancreatic development in vivo. Retroviral expression of PDX-1 in pancreatic cells induced the transcription of LRH-1, whereas reduced PDX-1 levels by RNA interference attenuated its expression. Consistent with direct regulation of LRH-1 expression by PDX-1, PDX-1(-/-) mice expressed smaller amounts of LRH-1 mRNA in the embryonic pancreas. Taken together, our data indicate that PDX-1 controls LRH-1 expression and identify LRH-1 as a novel downstream target in the PDX-1 regulatory cascade governing pancreatic development, differentiation, and function.
Place, publisher, year, edition, pages
2003. Vol. 23, no 19, 6713-6124 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-33518DOI: 10.1128/MCB.23.19.6713-6724.2003PubMedID: 12972592OAI: oai:DiVA.org:umu-33518DiVA: diva2:314048