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Determination of melamine in milk powder using zwitterionic HILIC stationary phase with UV detection
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2010 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 33, no 6-7, 988-995 p.Article in journal (Refereed) Published
Abstract [en]

A hydrophilic interaction liquid chromatography-UV method was developed for the determination of melamine in milk powder. Sulfobetaine type zwitterionic hydrophilic interaction liquid chromatography stationary phase was used to achieve straightforward separation of melamine in milk powder without any sample derivatization or addition of ion-pair reagent. The sample preparation was simple and fast with the steps of acetonitrile/perchloric acid extraction-centrifugation-filtration. No SPE or other pre-concentration procedure was required. By using large volume sample injection and choosing low UV wavelength (210 nm), the LOD and LOQ were 0.005 and 0.015 mug/mL for melamine standards, and 0.02 and 0.06 mug/mL for the spiked milk extracts. The LOD and LOQ of the latter correspond to 0.95 and 2.2 mug/g melamine in the milk powder. The correlation coefficients for melamine standard, pre-spiked milk extracts and post-spiked milk extracts in the range of 0-0.5 mug/mL were 0.9978, 0.9976 and 0.9995, respectively. This newly developed method is sensitive and cost effective, therefore, suitable for screening of melamine in tainted milk products.

Place, publisher, year, edition, pages
2010. Vol. 33, no 6-7, 988-995 p.
Keyword [en]
hydrophilic interaction liquid chromatography, HILIC, melamine analysis, milk powder, perchloric acid, UV
National Category
Chemical Sciences
URN: urn:nbn:se:umu:diva-33672DOI: 10.1002/jssc.200900815ISI: 000276782700034PubMedID: 20209504OAI: diva2:317097
Available from: 2010-05-03 Created: 2010-05-03 Last updated: 2011-12-21Bibliographically approved

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Nkem Ihunegbo, FeliciaTesfalidet, Solomon
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