POF Regulates the Expression of Genes on the Fourth Chromosome in Drosophila melanogaster by Binding to Nascent RNA
2012 (English)In: Molecular and Cellular Biology, ISSN 0270-7306, E-ISSN 1098-5549, Vol. 32, no 11, 2121-2134 p.Article in journal (Other academic) Published
In Drosophila, two chromosome-wide compensatory systems have been characterized: the dosage compensation system that acts on the male X chromosome and the chromosome-specific regulation of genes located on the heterochromatic fourth chromosome. Dosage compensation in Drosophila is accomplished by hypertranscription of the single male X chromosome mediated by the male-specific lethal (MSL) complex. The mechanism of this compensation is suggested to involve enhanced transcriptional elongation mediated by the MSL complex, while the mechanism of compensation mediated by the painting of fourth (POF) protein on the fourth chromosome has remained elusive. Here, we show that POF binds to nascent RNA, and this binding is associated with increased transcription output from chromosome 4. We also show that genes located in heterochromatic regions spend less time in transition from the site of transcription to the nuclear envelope. These results provide useful insights into the means by which genes in heterochromatic regions can overcome the repressive influence of their hostile environment.
Place, publisher, year, edition, pages
American Society for Microbiology , 2012. Vol. 32, no 11, 2121-2134 p.
Genetics Biochemistry and Molecular Biology
Research subject Genetics
IdentifiersURN: urn:nbn:se:umu:diva-33925DOI: 10.1128/MCB.06622-11ISI: 000304293000007PubMedID: 22473994OAI: oai:DiVA.org:umu-33925DiVA: diva2:318761
First published in thesis in manuscript form with title: "POF regulates the 4th chromosome through association to nascent RNA"2010-05-102010-05-102016-08-29Bibliographically approved