Calcium-regulated type III secretion of Yop proteins by an Escherichia coli hha mutant carrying a Yersinia pestis pCD1 virulence plasmid.
2006 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 74, no 2, 1381-6 p.Article in journal (Refereed) Published
A series of four large deletions that removed a total of ca. 36 kb of DNA from the ca. 70-kb Yersinia pestis pCD1 virulence plasmid were constructed using lambda Red-mediated recombination. Escherichia coli hha deletion mutants carrying the virulence plasmid with the deletions expressed a functional calcium-regulated type III secretion system. The E. coli hha/pCD1 system should facilitate molecular studies of the type III secretion process.
Place, publisher, year, edition, pages
2006. Vol. 74, no 2, 1381-6 p.
IdentifiersURN: urn:nbn:se:umu:diva-33950DOI: 10.1128/IAI.74.2.1381-1386.2006PubMedID: 16428789OAI: oai:DiVA.org:umu-33950DiVA: diva2:318870