Intracellular survival of Yersinia spp. in macrophages requires outer membrane protein A (OmpA)
(English)Manuscript (preprint) (Other academic)
In order to grow, multiply and disseminate within their hosts, Yersinia spp. must be able tosurvive encounters with host phagocytic cells such as macrophages. Yersinia pestis and Yersinia pseudotuberculosis both have the ability to survive within the phagolysosome of macrophages which may be important in early stages of the infection. These organisms also employ a T3SS toactively prevent phagocytosis. The ability of Y. pestis KIM mutants defective in the expression of outer membrane proteins to survive and proliferate in the presence of RAW 264.7 macrophage-like cells was evaluated. A Y. pestis KIM ΔompA mutant exhibited a significant defect in proliferation in the presence of macrophages. A similar defect could be produced by overexpressing the negative regulatory sRNA MicA, which down regulates OmpA expression. The ΔompA mutant exhibited no defect in the T3SS-mediated secretion or injection of Yops; however, this mutant showed a defect in intra macrophage survival in gentamicin protection assays. These studies suggest that the Yersinia spp. OmpA protein is a virulence factor that is required for survival within macrophages.
Microbiology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-33952OAI: oai:DiVA.org:umu-33952DiVA: diva2:318876