Change search
ReferencesLink to record
Permanent link

Direct link
Plasma brominated polymer particles as grafting substrate for thiol-terminated telomers
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Show others and affiliations
2010 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 33, no 11, 1563-70 p.Article in journal (Refereed) Published
Abstract [en]

A combined surface activation and "grafting to" strategy was developed to convert divinylbenzene particles into weak cation exchangers suitable for protein separation. The initial activation step was based on plasma modification with bromoform, which rendered the particles amenable to further reaction with nucleophiles by introducing Br to a surface content of 11.2 atom-%, as determined by X-ray photoelectron spectroscopy. Grafting of thiol-terminated glydicyl methacrylate telomers to freshly plasma activated surfaces was accomplished without the use of added initiator, and the grafting was verified both by reduction in bromine content and the appearance of sulfur-carbon linkages, showing that the surface grafts were covalently bonded. Following grafting the attached glydicyl methacrylate telomer tentacles were further modified by a two-step procedure involving hydrolysis to 2,3-hydroxypropyl groups and conversion of hydroxyl groups to carboxylate functionality by succinic anhydride. The final material was capable of baseline separating four model proteins in 3 min by gradient cation exchange chromatography in a fully aqueous eluent.

Place, publisher, year, edition, pages
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA , 2010. Vol. 33, no 11, 1563-70 p.
Keyword [en]
Grafting to, Non-porous divinylbenzene particles, Plasma bromination, Precipitation polymerization, Telomer surface modification
URN: urn:nbn:se:umu:diva-34218DOI: 10.1002/jssc.201000028ISI: 000278829700005PubMedID: 20432486OAI: diva2:320036
Available from: 2010-05-21 Created: 2010-05-21 Last updated: 2010-06-07Bibliographically approved
In thesis
1. Porous polymeric materials for chromatography: Synthesis, functionalization and characterization
Open this publication in new window or tab >>Porous polymeric materials for chromatography: Synthesis, functionalization and characterization
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Background: Separation science is heavily reliant on materials to fulfill ever more complicated demands raised by other areas of science, notably the rapidly expanding molecular biosciences and environmental monitoring. The key to successful separations lies in a combination of physical properties and surface chemistry of stationary phases used in liquid chromatographic separation, and this thesis address both aspects of novel separation materials.

Methods: The thesis accounts for several approaches taken during the course of my graduate studies, and the main approaches have been i) to test a wild-grown variety of published methods for surface treatment of fused silica capillaries, to ascertain firm attachment of polymeric monoliths to the wall of microcolumns prepared in silica conduits; ii) developing a novel porogen scheme for organic monoliths including polymeric porogens and macromonomers; iii) evaluating a mesoporous styrenic monolith for characterization of telomers intended for use in surface modification schemes and; iv) to critically assess the validity of a common shortcut used for estimating the porosity of monoliths prepared in microconduits; and finally v) employing plasma chemistry for activating and subsequently modifying the surface of rigid, monodisperse particles prepared from divinylbenzene.

Results: The efforts accounted for above have resulted in i) better knowledge of the etching and functionalization parameters that determine attachment of organic monoliths prepared by radical polymerization to the surface of silica; ii) polar methacrylic monoliths with a designed macroporosity that approaches the desired "connected rod" macropore morphology; iii) estab¬lishing the usefulness of monoliths prepared via nitroxide mediated polymerization in gradient polymer elution chromatography; iv) proving that scanning electron microscopy images are of limited value for assessing the macroporous properties of organic monoliths, and that pore measurements on externally polymerized monolith cocktails do not represent the porous properties of the same cocktail polymerized in narrow confinements; and v) showing that plasma bromination can be used as an activation step for rigid divinylbenzene particles to act as grafting handles for epoxy-containing telomers, that can be attached in a sufficiently dense layer and converted into carboxylate cation exchange layer that allows protein separations in fully aqueous eluents.

Place, publisher, year, edition, pages
Umeå: Kemiska Institutionen, Umeå universitet, 2009. 45 p.
porous polymer monolith, pore size distribution, nitrogen sorptiometry, mercury intrusion porosimetry, scanning and transmission electron microscopy, plasma functionalization
National Category
Analytical Chemistry Analytical Chemistry Polymer Chemistry
Research subject
Analytical Chemistry
urn:nbn:se:umu:diva-30314 (URN)978-91-7264-934-7 (ISBN)
Public defence
2010-01-15, KBC-Huset, KB3A9, Umeå Universitet, Umeå, 13:00 (English)
Available from: 2009-12-18 Created: 2009-12-16 Last updated: 2010-06-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Dinh, Ngoc PhuocIrgum, Knut
By organisation
Department of Chemistry
In the same journal
Journal of Separation Science

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 178 hits
ReferencesLink to record
Permanent link

Direct link