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Effect of nitric oxide donors on membrane tritium accumulation of endocannabinoids and related endogenous lipids.
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Pharmacology.
2009 (English)In: European Journal of Pharmacology, ISSN 0014-2999, E-ISSN 1879-0712, Vol. 621, no 1-3, 10-18 p.Article in journal (Refereed) Published
Abstract [en]

The endocannabinoids anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are metabolised by cells by hydrolysis to arachidonic acid followed by esterification into phospholipids. Here, we report that nitric oxide (NO) donors significantly increase the amount of tritium accumulated in the cell membranes of RBL2H3 rat basophilic cells, 3T3-L1 mouse fibroblast cells and b.End5 mouse brain endothelioma cells following incubation of the intact cells with AEA labelled in the arachidonate part of the molecule. Similar results were seen with 2-AG and with arachidonic acid, whilst the NO donors reduced the accumulation of tritium after incubation of RBL2H3 cells with AEA labelled in the ethanolamine part of the molecule. Pretreatment of intact cells with NO donors did not increase the activity of the enzyme mainly responsible for metabolism of AEA, fatty acid amide hydrolase (FAAH). Furthermore, inhibition of FAAH completely blocked the effect produced by NO donors in cells with a large FAAH component, suggesting that for AEA, the effects were downstream of the enzyme. These data raise the possibility that the cellular processing of endocannabinoids following its uptake can be regulated by nitric oxide.

Place, publisher, year, edition, pages
2009. Vol. 621, no 1-3, 10-18 p.
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:umu:diva-34658DOI: 10.1016/j.ejphar.2009.08.028ISI: 000271153000002PubMedID: 19715690OAI: oai:DiVA.org:umu-34658DiVA: diva2:323369
Available from: 2010-06-10 Created: 2010-06-10 Last updated: 2012-07-04Bibliographically approved

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Thors, LinaFowler, Christopher J

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