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In vitro effect of ultrasound on bacteria and suggested protocol for sonication and diagnosis of prosthetic infections
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Orthopaedics.
2009 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 47, no 8, 2496-2501 p.Article in journal (Refereed) Published
Abstract [en]

Sonication of implants has been shown to be a promising method for diagnosis of prosthetic infections due to its improved sensitivity, simplicity, and low cost. The aim of the present study was to evaluate the effects of ultrasound performed under different conditions regarding temperature, duration, and composition of sonication tubes on bacterial species often associated with prosthetic infections. We found that ultrasound had an inhibitory effect on bacteria, of which gram-negative bacteria, in particular Escherichia coli, were almost eradicated after 5 min of sonication at 35 degrees C. Gram-positive bacteria were found to be resistant to the effect of ultrasound. Four factors were important for the inhibitory effect of sonication: the type of microorganism, the temperature of the sonication buffer, the duration of exposure to ultrasound (minutes), and the material and composition of the sonication tube in which sonication is performed. On the basis of the results from the present study, we propose a protocol for sonication and recovery of bacteria associated with biofilm on infected implants prior to conventional culture. From the present protocol, we recommend sonication for 7 min at 22 degrees C at the maximum effect which permits survival of gram-negative bacteria.

Place, publisher, year, edition, pages
2009. Vol. 47, no 8, 2496-2501 p.
Keyword [en]
small-colony variants; orthopedic implants; joint infections; hip; colonization; recovery; devices; risk
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-35115DOI: 10.1128/JCM.02316-08PubMedID: 19535525OAI: oai:DiVA.org:umu-35115DiVA: diva2:329597
Available from: 2010-07-12 Created: 2010-07-12 Last updated: 2017-12-12Bibliographically approved

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Monsen, TorLövgren, ElisabethWiderström, MicaelWallinder, Lars
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