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Surface characterization of bone graft substitute materials conditioned in cell culture medium
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Medicine, Department of Odontology.
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2010 (English)In: Surface and Interface Analysis, ISSN 0142-2421, E-ISSN 1096-9918, Vol. 42, no 6-7, 452-456 p.Article in journal (Refereed) Published
Abstract [en]

Biomaterials are widely used in clinical practice as bone graft substitutes for treating patients with bone defects. A molecular level understanding of the chemical processes at the interface between the biomaterial and the biological environment is crucial to succeed in tissue regeneration and to predict the treatment outcome. In this study, we used three different bone graft substitute materials (BioGlass 45S5—synthetic, Bio-Oss—bovine derived and Algipore—derived from algae) which were incubated in an α-minimum essential medium (α-MEM) during 1, 3 and 7 days. Initial surface composition of the biomaterials and the chemistry of their solid–solution interface were monitored by XPS with a fast-frozen samples technique. The XPS analysis showed that the equilibrium at the solid-solution interface is reached within 24 h. The Na/Cl atomic ratio at equilibrium indicates a negatively charged surface for Bio-Oss. In contrast, the other two materials gained a positive surface charge, which resulted in pronounced adsorption of amino acids at the interface from the medium. The surface chemical reconstruction and charge generation mechanism responsible for this effect are discussed with regard to bulk composition of the materials and possible proliferation and differentiation cell patterns that could be expected at the interface. Copyright © 2010 John Wiley & Sons, Ltd.

Place, publisher, year, edition, pages
John Wiley & Sons, Ltd , 2010. Vol. 42, no 6-7, 452-456 p.
Keyword [en]
XPS, solid–liquid interface, bone graft substitute, biomaterial, Bio-Oss, Algipore, Bioglass 45S5
National Category
Dentistry
Identifiers
URN: urn:nbn:se:umu:diva-35478DOI: 10.1002/sia.3337ISI: 000281149700004OAI: oai:DiVA.org:umu-35478DiVA: diva2:344476
Available from: 2010-08-19 Created: 2010-08-19 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Biological interface of bone graft substitute materials: experimental studies on interactions between biomaterials and bone cells
Open this publication in new window or tab >>Biological interface of bone graft substitute materials: experimental studies on interactions between biomaterials and bone cells
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bone graft substitute materials are becoming more common as alternative therapy strategies when bone defects in patients need to be treated. The interaction between bone cells and biomaterials occur at the surface of the materials. A great deal is known about the importance of surface topography and physic-chemical properties of biomaterials. It is also known that cells require proteins in order to interact with biomaterials. Less is known about how material properties and proteins interact forming the biological interface that cells will be exposed to, and that might determine if new bone is formed or not in the patient. The overall aim of the present thesis was to systematically investigate bone graft substitute material surface reactions and the interface in order to better understand how biomaterials may promote bone formation. Bio-Oss (BO) is a commonly used bone graft substitute material in reconstruction of periodontal and dentoalveolar bone defects. BO is mainly considered to be “osteoconductive”, but we could show that it does interact with a biological fluid (α-MEM cell culture medium) through dissolution/precipitation reactions. A significant reduction of calcium and phosphate levels in the medium was obtained even with low concentrations of BO. A release of silicon from the material was also demonstrated. An osteogenic response was seen in close contact to the BO particles when cultured with different types of pre-osteoblastic cells (Paper I). X-Ray Photoelectron Spectroscopy (XPS) with fast-frozen sample technique was used to further characterize the surface of BO, Frios Algipore (AP) and 45S5 Bioglass (BG). These three bone graft substitute materials are used as “model systems”, because they have all demonstrated newly formed bone on the surface after implantation in patients. From the XPS analysis it can be concluded that AP and BG acquired a positively charged surface while BO gained a negatively charged surface. Only AP and BG adsorb organic components (amino acids) from the medium (Paper II). Next we investigated initial surface reactions and the formation of a biological interface in the presence of proteins (serum) for the three biomaterials. The major findings were that in the presence of proteins BO underwent a surface charge reversal, all three biomaterials adsorbed proteins on the surface and all three biomaterials altered the chemical composition of the cell culture medium (Paper III-IV). Silicon (Si), which was released from BO as well as from BG, is interesting in relation to bone health. Positive effects of BG Si dissolution products on osteoblasts have been reported earlier. In the present study inhibitory interactions of Si on the RANK/RANKL/OPG signaling pathway as well as with gap junction intercellular communication in vitro are reported. These new findings implicate that Si could potentially be beneficial for patients with imbalance in bone remodeling (osteoporosis) and treatments of bone defects (Paper V). In conclusion, biomaterials of different origins interact with a solution resembling the extracellular tissue fluid. The dissolution-precipitation reactions are influenced by the material concentration used and should be taken into consideration when designing experiments and when biomaterials are used clinically. The presence of proteins will influence surface reactions, the formation of the biological interface and have implications on cellular responses. Possible dissolution products from the biomaterials should be investigated.  Si, a dissolution product, is shown to have an inhibitory effect on osteoclastogenesis and bone resorption in vitro. Potential clinical value of Si in treatment of patients with bone defects should be further investigated.

Place, publisher, year, edition, pages
Umeå: Umeå university, 2011. 40 p.
Series
Umeå University odontological dissertations, ISSN 0345-7532 ; 121
National Category
Dentistry
Identifiers
urn:nbn:se:umu:diva-49818 (URN)978-91-7459-286-3 (ISBN)
Public defence
2011-12-09, Sal B, by1 D 9tr, Tandläkarhögskolan, Norrlands universitetsjukhus, Umeå, 13:00 (English)
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Available from: 2011-11-21 Created: 2011-11-18 Last updated: 2011-11-21Bibliographically approved

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Mladenovic, ZivkoSahlin-Platt, AnnikaRansjö, MariaShchukarev, Andrey

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Mladenovic, ZivkoSahlin-Platt, AnnikaBengtsson, ÅsaRansjö, MariaShchukarev, Andrey
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Surface and Interface Analysis
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