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Biochemical and functional characterization of Helicobacter pylori vesicles
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. Umeå University, Faculty of Medicine, Department of Odontology, Oral Microbiology.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
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2010 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 77, no 6, 1539-1555 p.Article in journal (Refereed) Published
Abstract [en]

Helicobacter pylori can cause peptic ulcer disease and/or gastric cancer. Adhesion of bacteria to the stomach mucosa is an important contributor to the vigor of infection and resulting virulence. H. pylori adheres primarily via binding of BabA adhesins to ABO/Lewis b (Leb) blood group antigens and the binding of SabA adhesins to sialyl-Lewis x/a (sLex/a) antigens. Similar to most Gram-negative bacteria, H. pylori continuously buds off vesicles and vesicles derived from pathogenic bacteria often include virulence-associated factors. Here we biochemically characterized highly purified H. pylori vesicles. Major protein and phospholipid components associated with the vesicles were identified with mass spectroscopy and NMR. A subset of virulence factors present was confirmed by immunoblots. Additional functional and biochemical analysis focused on the vesicle BabA and SabA adhesins and their respective interactions to human gastric epithelium. Vesicles exhibit heterogeneity in their protein composition, which were specifically studied in respect to the BabA adhesin. We also demonstrate that the oncoprotein, CagA, is associated with the surface of H. pylori vesicles. Thus, we have explored mechanisms for intimate H. pylori vesicle-host interactions and found that the vesicles carry effector-promoting properties that are important to disease development.

Place, publisher, year, edition, pages
Wiley , 2010. Vol. 77, no 6, 1539-1555 p.
Identifiers
URN: urn:nbn:se:umu:diva-35586DOI: 10.1111/j.1365-2958.2010.07307.xISI: 000281831400018PubMedID: 20659286OAI: oai:DiVA.org:umu-35586DiVA: diva2:345231
Available from: 2010-08-24 Created: 2010-08-24 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Helicobacter pylori outer membrane vesicles and the host-pathogen interaction
Open this publication in new window or tab >>Helicobacter pylori outer membrane vesicles and the host-pathogen interaction
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Helicobacter pylori membranvesiklar och interaktioner med värdcellen
Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2013. 56 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1559
Keyword
H. pylori, outer membrane vesicles, 2D 31P, 1H COSY NMR, phospholipids, OMV proteome, adherence, endocytosis, CagA, OMV-host cell responses, yttermembran vesiklar
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:umu:diva-68744 (URN)978-91-7459-578-9 (ISBN)
Public defence
2013-05-17, KB3B1, KBC-huset, Umeå universitet, Umeå, 09:00 (English)
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Available from: 2013-04-26 Created: 2013-04-24 Last updated: 2015-11-17Bibliographically approved

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Olofsson, AnnelieVallström, AnnaPetzold, KatjaSchleucher, JürgenCarlsson, SvenWai, Sun NyuntGröbner, GerhardArnqvist, Anna
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Department of Medical Biochemistry and BiophysicsOral MicrobiologyDepartment of Molecular Biology (Faculty of Medicine)Department of Chemistry
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Molecular Microbiology

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