Electrochemical impedance spectroscopy in label-free biosensor applications: multivariate data analysis for an objective interpretation
2010 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 398, no 6, 2341-9 p.Article in journal (Refereed) Published
Electrochemical impedance spectroscopy plays an important role in biosensor science thanks to the possibility of finding specific information from processes with different kinetics at a chosen electrode potential in one experiment. In this paper we briefly discuss label-free impedimetric biosensors described in the literature. A novel method for neutral interpretation of impedance data is presented that includes complex number chemometrics. Three examples are given based on impedance measurements on synthetic biomembranes, in this case a lipid monolayer deposited on a mercury electrode. The interaction of various compounds with the monomolecular lipid layer is illustrated with the following: (1) different concentrations of magainin (Geladi et al. in Proc. Int. Fed. Med. Biomed. Eng. 9:219-220, 2005); (2) different derivatives of gramicidin A (Lindholm-Sethson et al. in Langmuir 24:5029-5032, 2007), and (3) an antimicrobial peptide (Ringstad et al. in Langmuir 24:208-216, 2008).
Place, publisher, year, edition, pages
Springer , 2010. Vol. 398, no 6, 2341-9 p.
Impedance, Biosensor, Complex number chemometrics
IdentifiersURN: urn:nbn:se:umu:diva-36186DOI: 10.1007/s00216-010-4027-7ISI: 000283588600002PubMedID: 20676616OAI: oai:DiVA.org:umu-36186DiVA: diva2:352546