umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The TL29 protein is lumen located, associated with PSII and not an ascorbate peroxidase
Umeå University, Faculty of Science and Technology, Department of Chemistry. (Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83 Umeå, Sweden)
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Department of Life Sciences, Södertörns University College, SE-141 89 Huddinge, Sweden.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Show others and affiliations
2009 (English)In: Plant and Cell Physiology, ISSN 0032-0781, E-ISSN 1471-9053, Vol. 50, no 11, 1898-1910 p.Article in journal (Refereed) Published
Abstract [en]

The TL29 protein is one of the more abundant proteins in the thylakoid lumen of plant chloroplasts. Based on its sequence homology to ascorbate peroxidases, but without any supporting biochemical evidence, TL29 was suggested to be involved in the plant defense system against reactive oxygen species and consequently renamed to APX4. Our in vivo and in vitro analyses failed to show any peroxidase activity associated with TL29; it bound neither heme nor ascorbate. Recombinant overexpressed TL29 had no ascorbate-dependent peroxidase activity, and various mutational analyses aiming to convert TL29 into an ascorbate peroxidase failed. Furthermore, in the thylakoid lumen no such activity could be associated with TL29 and, additionally, TL29 knock-out mutants did not show any decreased peroxidase activity or increased content of radical oxygen species when grown under light stress. Instead we could show that TL29 is a lumen-located component associated with PSII.

Place, publisher, year, edition, pages
Oxford Journals , 2009. Vol. 50, no 11, 1898-1910 p.
Keyword [en]
APX4, Arabidopsis, Heme, Lumen, Thylakoid membrane
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:umu:diva-36566DOI: 10.1093/pcp/pcp134PubMedID: 19828564OAI: oai:DiVA.org:umu-36566DiVA: diva2:354737
Available from: 2010-10-04 Created: 2010-10-04 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Granlund, IreneStorm, PatrikGarcía-Cerdán, José GFunk, ChristianeSchröder, Wolfgang P
By organisation
Department of ChemistryUmeå Plant Science Centre (UPSC)
In the same journal
Plant and Cell Physiology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 153 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf