Difference gel electrophoresis (DIGE)
2010 (English)In: Encyclopedia of Life Sciences, Wiley , 2010Chapter in book (Other (popular science, discussion, etc.))
One of the largest challenges in proteomics today is to be able to quantify the composition and amount of proteins found in a specific cell or tissue at a defined time point. Difference gel electrophoresis (DIGE) is a gel electrophoresis-based technique for protein quantification in complex mixtures. In DIGE the high resolution of two-dimensional gel electrophoresis is combined with the excellent dynamic range obtained by fluorescent tag labelling of protein samples. The output of DIGE experiments provides information about how many proteins display changed expression levels on a specific treatment. In addition, proteins of interest can be excised and identified with conventional mass spectrometry techniques and further analysed by other biochemical methods.
Place, publisher, year, edition, pages
Wiley , 2010.
DIGE, 2D-PAGE, CyDye, protein expression, fluorescence
IdentifiersURN: urn:nbn:se:umu:diva-36567DOI: 10.1002/9780470015902.a0021881OAI: oai:DiVA.org:umu-36567DiVA: diva2:354790
Published Online 15 December 20092010-10-042010-10-042011-10-14Bibliographically approved