The neuroblastoma ALK(I1250T) mutation is a kinase-dead RTK in vitro and in vivo
2011 (English)In: Translational Oncology, ISSN 1936-5233, Vol. 4, no 4, 258-265 p.Article in journal (Other academic) Published
Activating mutations in the kinase domain of anaplastic lymphoma kinase (ALK) have recently been shown to be an important determinant in the genetics of the childhood tumor neuroblastoma. Here we discuss an in-depth analysis of one of the reported gain-of-function ALK mutations—ALKI1250T—identified in the germ line DNA of one patient. Our analyses were performed in cell culture-based systems and subsequently confirmed in a Drosophila model. The results presented here indicate that the germ line ALKI1250T mutation is most probably not a determinant for tumor initiation or progression and, in contrast, seems to generate a kinase-dead mutation in the ALK receptor tyrosine kinase (RTK). Consistent with this, stimulation with agonist ALK antibodies fails to lead to stimulation of ALKI1250T and we were unable to detect tyrosine phosphorylation under any circumstances. In agreement, ALKI1250T is unable to activate downstream signaling pathways or to mediate neurite outgrowth, in contrast to the activated wild-type ALK receptor or the activating ALKF1174S mutant. Identical results were obtained when the ALKI1250T mutant was expressed in a Drosophila model, confirming the lack of activity of this mutant ALK RTK. We suggest that the ALKI1250T mutation leads to a kinase-dead ALK RTK, in stark contrast to assumed gain-of-function status, with significant implications for patients reported to carry this particular ALK mutation.
Place, publisher, year, edition, pages
2011. Vol. 4, no 4, 258-265 p.
ALK, neuroblastoma, gain of function, kinase dead
Cell and Molecular Biology
Research subject Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-36979PubMedID: 21804922OAI: oai:DiVA.org:umu-36979DiVA: diva2:356947
ProjectsExploiting Drosophila as a model system for studying Anaplastic Lymphoma Kinase in vivo