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Spontaneous hair-cell renewal following gentamicin exposure in postnatal rat utricular explants
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Otorhinolaryngology.
Department of Otolaryngology, Albert Einstein College of Medicine, New York, NY.
Department of Otolaryngology, Albert Einstein College of Medicine, New York, NY.
Cochlear Implant Research Program, Department of Otolaryngology, University of Miami Ear Institute, University of Miami, School of Medicine, 1600 N.W. 10th Avenue, RMSB 3160, Miami.
2003 (English)In: Hearing Research, ISSN 0378-5955, E-ISSN 1878-5891, Vol. 180, no 1-2, 114-125 p.Article in journal (Refereed) Published
Abstract [en]

We have established an in vitro model of long-time culture of 4-day-old rat utricular maculae to study aminoglycoside-induced vestibular hair-cell renewal in the mammalian inner ear. The explanted maculae were cultured for up to 28 days on the surface of a membrane insert system. In an initial series of experiments utricles were exposed to 1 mM of gentamicin for 48 h and then allowed to recover in unsupplemented medium or in medium supplemented with the anti-mitotic drug aphidicolin. In a parallel control series, explants were not exposed to gentamicin. Utricles were harvested at specified time points from the second through the 28th day in vitro. Whole-mount utricles were stained with phalloidin-fluorescein isothiocyanate and their stereociliary bundles visualized and counted. In a second experimental series 2'-bromo-5'deoxyuridine labeling was used to confirm the antimitotic efficacy of aphidicolin. Loss of hair-cell stereociliary bundles was nearly complete 3 days after exposure to gentamicin, with the density of stereociliary bundles only 3-4% of their original density. Renewal of hair-cell bundles was abundant (i.e. 15x increase) in cultures in unsupplemented medium, with a peak of stereociliary bundle renewal reached after 21 days in vitro. A limited amount of hair-cell renewal also occurred in the presence of the anti-mitotic drug, aphidicolin. These results suggest that spontaneous renewal of hair-cell stereociliary bundles following gentamicin damage in utricular explants predominantly follows a pathway that includes mitotic events, but that a small portion of the hair-cell stereociliary bundle renewal does not require mitotic activity.

Place, publisher, year, edition, pages
2003. Vol. 180, no 1-2, 114-125 p.
Keyword [en]
In vitro model; Aminoglycoside; Vestibular hair-cell renewal; Mitotic activity
URN: urn:nbn:se:umu:diva-37205PubMedID: 12782359OAI: diva2:358288
Available from: 2010-10-21 Created: 2010-10-21 Last updated: 2010-12-06Bibliographically approved

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