Change search
ReferencesLink to record
Permanent link

Direct link
Myringotomy delays the tympanic membrane recovery in acute otitis media: a study in the rat model
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Otorhinolaryngology.
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Otorhinolaryngology.
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Otorhinolaryngology.
Department of Otorhinolaryngology, University of Porto Medical School Hospital, Porto, Portugal.
2002 (English)In: The Laryngoscope, ISSN 0023-852X, E-ISSN 1531-4995, Vol. 112, no 8 Pt 1, 1474-1481 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND/HYPOTHESIS: Acute otitis media is a major cause of visits to pediatric health care providers. Myringotomy in uncomplicated acute otitis media is debatable today. The study addressed this problem through the otomicroscopic and histopathological observations of the events occurring in the tympanic membrane during the first week after myringotomy.

STUDY DESIGN: Randomized study in an experimental animal model.

METHODS: Under anesthesia, the left middle ear of 36 Sprague-Dawley rats was inoculated with Streptococcus pneumoniae type 3. Forty-eight hours later, at day 0, four animals were immediately killed and the remaining animals were randomly assigned into a myringotomy group (n = 16, myringotomy on the left ear) and a non-myringotomy group (n = 16, otomicroscopy without myringotomy). Otomicroscopy and killings were performed in series of four animals from each group at days 1, 2, 4, and 7 after myringotomy. Tympanic membranes were collected after fixation and processed for light and electron microscopy.

RESULTS: All inoculated ears showed a manifest acute otitis media at day 0. An intense infiltration by inflammatory cells and edema distorted severely the tympanic membrane structure. These findings decreased the following days. However, inflammation as evaluated by the thickness and the cytoarchitecture of the tympanic membrane layers, recovered significantly faster in the membranes in the non-myringotomy group. At day 7, all tympanic membranes in the myringotomy group were closed by a hypertrophic keratinizing epithelium and a remodeling connective tissue layer, whereas the animals in the non-myringotomy group had a residual edema in the lamina propria.

CONCLUSIONS: The present infectious model induced an intense inflammatory reaction within the entire structure of the tympanic membrane. Myringotomy provoked a delayed recovery from the inflammatory process within the tympanic membrane. Therefore, if applicable to human conditions, the use of myringotomy in the management of acute otitis media should be restricted to selected cases of acute otitis media.

Place, publisher, year, edition, pages
2002. Vol. 112, no 8 Pt 1, 1474-1481 p.
Keyword [en]
Otitis media;tympanic membrane;myringotomy;rat;Streptococcus pneumoniae
URN: urn:nbn:se:umu:diva-37257DOI: 10.1097/00005537-200208000-00027PubMedID: 12172265OAI: diva2:358611
Available from: 2010-10-22 Created: 2010-10-22 Last updated: 2010-12-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Eriksson, Per-Olof
By organisation
In the same journal
The Laryngoscope

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 23 hits
ReferencesLink to record
Permanent link

Direct link