Tris(hydroxymethyl)aminomethane-functionalized silica particles and their application for hydrophilic interaction chromatography
2010 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 33, no 19, 2965-2976 p.Article in journal (Refereed) Published
A new method is presented for synthesizing a highly hydrophilic silica-based material for use in hydrophilic interaction chromatography. Porous silica particles used as a starting substrate were modified with 3-bromopropyl trichlorosilane and grafted with glycidyl methacrylate by controlled (“living”) atom transfer radical polymerization in order to introduce an oxirane-carrying reactive tentacle layer on the silica surface. The grafted material was thereafter subject to an oxirane ring opening reaction with tris(hydroxy-methyl)aminomethane in dimethylformamide to yield a polymer-bound equivalent of the well known and highly hydrophilic “TRIS” buffering substance. Chemical characterization was done by diffuse reflectance FT-IR, X-ray photoelectron spectroscopy, elemental analysis, and 1H NMR. Porosity and surface area examination was done with Brunauer–Emmett–Teller. Chromatographic application of the material was evaluated by separations of nucleic bases, small organic acids, and common nucleotides under mixed hydrophilic interaction chromatography and weak anion exchange conditions.
Place, publisher, year, edition, pages
John Wiley & Sons, 2010. Vol. 33, no 19, 2965-2976 p.
Hydrophilic interaction chromatography, Silica, Trishydroxymethyl aminomethane
IdentifiersURN: urn:nbn:se:umu:diva-37267DOI: 10.1002/jssc.201000154ISI: 000283201900007OAI: oai:DiVA.org:umu-37267DiVA: diva2:358730