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A metabolomic approach to study major metabolite changes during acclimation to limiting CO2 in chlamydomonas reinhardtii
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2010 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 154, no 1, 187-196 p.Article in journal (Refereed) Published
Abstract [en]

Using a gas chromatography-mass spectrometry-time of flight technique, we determined major metabolite changes during induction of the carbon-concentrating mechanism in the unicellular green alga Chlamydomonas reinhardtii. In total, 128 metabolites with significant differences between high-and low-CO2-grown cells were detected, of which 82 were wholly or partially identified, including amino acids, lipids, and carbohydrates. In a 24-h time course experiment, we show that the amino acids serine and phenylalanine increase transiently while aspartate and glutamate decrease after transfer to low CO2. The biggest differences were typically observed 3 h after transfer to low-CO2 conditions. Therefore, we made a careful metabolomic examination at the 3-h time point, comparing low-CO2 treatment to high-CO2 control. Five metabolites involved in photorespiration, 11 amino acids, and one lipid were increased, while six amino acids and, interestingly, 21 lipids were significantly lower. Our conclusion is that the metabolic pattern during early induction of the carbon-concentrating mechanism fit a model where photorespiration is increasing.

Place, publisher, year, edition, pages
2010. Vol. 154, no 1, 187-196 p.
Identifiers
URN: urn:nbn:se:umu:diva-37355DOI: 10.1104/pp.110.157651ISI: 000281570000015OAI: oai:DiVA.org:umu-37355DiVA: diva2:359497
Available from: 2010-10-28 Created: 2010-10-28 Last updated: 2017-12-12Bibliographically approved

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Renberg, LindaShutova, TatianaStenlund, HansAksmann, AnnaGardeström, PerMoritz, ThomasSamuelsson, Göran
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Umeå Plant Science Centre (UPSC)Department of Plant PhysiologyDepartment of Chemistry
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CiteExportLink to record
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