Modification of Xylan-lignin interactions by targeting novel fungal enzymes to the wood cell wall
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Xylan is an abundant constituent of hemicelluloses. It has a complex structure and is bound to lignin via covalent bonds. These bonds and xylan structure can be altered to modify cell wall properties using various enzymes. These enzymes can be obtained from different organisms. Certain wood degrading fungi have been reported to produce enzymes acting on xylan such as xylanases or α-glucuronidases. We aimed to target these enzymes to the secondary cell wall of plants. This was achieved by fusing the enzyme coding sequence with plant signal sequence. The targeting was verified by analyzing the localization of the target protein with the fluorescent tag GFP.
Place, publisher, year, edition, pages
2010. , 15 p.
IdentifiersURN: urn:nbn:se:umu:diva-37359OAI: oai:DiVA.org:umu-37359DiVA: diva2:359514
UppsokLife Earth Science
Mellerowicz, Ewa, Prof