The platinum and copper metallome from sensitive and resistant T289 melanoma cells exposed to Cisplatin studied by a multi-analytical approach
(English)Manuscript (preprint) (Other academic)
The copper and platinum metallome were evaluated in cisplatin (CDDP) sensitive and resistant melanoma T289 cells after exposure to cisplatin. Determination of the total concentrations of copper and platinum were combined with analysis of the intracellular copper and platinum profiles acquired by size exclusion chromatography (SEC) and reversed phase liquid chromatography (RPLC) coupled to inductively coupled plasma and electrospray ionization mass spectrometry (ICPMS and ESIMS, respectively). The total concentration of platinum in sensitive cells was 50-80% higher than in the resistant cell line and the intracellular copper concentration was decreased by ~30% when exposing the cells to cisplatin. SEC-ICPMS and RPLC-ICPMS profiles showed no obvious differences in the platinum profile between sensitive and resistant cells. However, the copper profiles differed showing a significant increase of some still unidentified copper species with an estimated molecular mass of 25-30 kDa. All together the data support the view that components of the copper homeostatic system are involved in CDDP accumulation and are affected by cisplatin exposure.
Metallome, HPLC-ICPMS, HPLC-ESIMS, Platinum, Copper
Research subject Analytical Chemistry
IdentifiersURN: urn:nbn:se:umu:diva-37395OAI: oai:DiVA.org:umu-37395DiVA: diva2:360169