umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The multifunctional GAP protein YopE of Yersinia is involved in effector translocation control and virulence
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Hans Wolf-Watz)
2010 (English)Doctoral thesis, comprehensive summary (Other academic)Alternative title
Det multifunktionella GAP proteinet YopE från Yersinia är involverat i kontroll av effektortranslokering och virulens (Swedish)
Abstract [en]

The Gram-negative bacterium Yersinia pseudotuberculosis employs a type 3 secretion system (T3SS) to establish infections. The T3SS translocates a diverse set of effector proteins directly into the host cells. The coordinate action of the translocated effectors blocks the innate immune system of the host and ensures extracellular proliferation of the bacterium. YopE is an essential effector that disrupts the actin cytoskeleton of infected host cells. This cytotoxicity is caused by the inactivation of RhoGTPases by the GTPase Activating Protein (GAP) activity of YopE. YopE was demonstrated to inactivate the RhoGTPases Rac1 and RhoA in vivo. However, Rac1 and RhoA inactivation was not a prerequisite for cytotoxicity or virulence. Thus, YopE must have additional targets during infection. Surprisingly, avirulent yopE mutants had lost the control of Yop expression in the presence of target cells and they all overtranslocated effectors. It appeared as if translocated YopE was able to control Yop expression and effector translocation via a feedback inhibition mechanism. This feedback inhibition was dependent on functional GAP activity. Translocation control could also be mediated by exogenous GAP activity, suggesting that effector translocation control might be a general property of all bacterial GAP proteins. Besides YopE, the regulatory protein YopK was also found to be involved in the effector translocation control process. Clearly, as demonstrated in virulence, the roles for YopE and YopK are intimately related.                       Further, YopE possesses a membrane localization domain (MLD) required for proper localization. A yopE∆MLD mutant had lost the feedback inhibition of YopE expression and was avirulent. Hence, the effector translocation control of YopE requires both proper localization as well as functional GAP activity.                                           In addition, fish keratocytes were established as a novel model system for Y. pseudotuberculosis infections. YopE was found to be the sole effector responsible for cytotoxicity towards the keratocytes. Further, induction of cytotoxicity required fully native YopE protein which indicated that the keratocytes would be useful as a sensitive model system for further studies of YopE mediated phenotypes.

In summary, this thesis work has sought to unravel the multiple functions of translocated YopE. A novel role was elucidated where Yersinia utilizes translocated YopE to control the process of effector translocation into host cells. This regulatory control was connected to virulence in the mouse model of disease. Thus, perhaps YopE should be considered also as a regulatory protein besides being a classical effector.

Place, publisher, year, edition, pages
Umeå: Umeå universitet , 2010. , 56 p.
Keyword [en]
Yersinia, T3SS, YopE, GAP activity, translocation control, virulence
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-37960ISBN: 978-91-7459-100-2 (print)OAI: oai:DiVA.org:umu-37960DiVA: diva2:371253
Public defence
2010-12-13, N320, Naturvetarhuset, Umeå universitet, Umeå, 10:00 (English)
Opponent
Supervisors
Available from: 2010-11-22 Created: 2010-11-19 Last updated: 2010-11-22Bibliographically approved
List of papers
1. Functional analysis of the YopE GTPase-activating protein (GAP) activity of Yersinia pseudotuberculosis
Open this publication in new window or tab >>Functional analysis of the YopE GTPase-activating protein (GAP) activity of Yersinia pseudotuberculosis
Show others...
2006 (English)In: Cellular Microbiology, ISSN 1462-5814, E-ISSN 1462-5822, Vol. 8, no 6, 1020-1033 p.Article in journal (Refereed) Published
Abstract [en]

YopE of Yersinia pseudotuberculosis inactivates three members of the small RhoGTPase family (RhoA, Rac1 and Cdc42) in vitro and mutation of a critical arginine abolishes both in vitro GTPase-activating protein (GAP) activity and cytotoxicity towards HeLa cells, and renders the pathogen avirulent in a mouse model. To understand the functional role of YopE, in vivo studies of the GAP activity in infected eukaryotic cells were conducted. Wild-type YopE inactivated Rac1 as early as 5 min after infection whereas RhoA was down regulated about 30 min after infection. No effect of YopE was found on the activation state of Cdc42 in Yersinia-infected cells. Single-amino-acid substitution mutants of YopE revealed two different phenotypes: (i) mutants with significantly lowered in vivo GAP activity towards RhoA and Rac1 displaying full virulence in mice, and (ii) avirulent mutants with wild-type in vivo GAP activity towards RhoA and Rac1. Our results show that Cdc42 is not an in vivo target for YopE and that YopE interacts preferentially with Rac1, and to a lesser extent with RhoA, during in vivo conditions. Surprisingly, we present results suggesting that these interactions are not a prerequisite to establish infection in mice. Finally, we show that avirulent yopE mutants translocate YopE in about sixfold higher amount compared with wild type. This raises the question whether YopE's primary function is to sense the level of translocation rather than being directly involved in downregulation of the host defence.

Place, publisher, year, edition, pages
Wiley, 2006
Keyword
Animals, Bacterial Outer Membrane Proteins/*analysis/genetics/*physiology, Bacterial Translocation/physiology, DNA; Bacterial/analysis/genetics, Down-Regulation/physiology, Female, GTPase-Activating Proteins/*analysis/*physiology, Gene Expression Regulation; Bacterial, Hela Cells, Humans, L-Lactate Dehydrogenase/metabolism, Mice, Mice; Inbred C57BL, Mutation, Substrate Specificity, Virulence, Yersinia pseudotuberculosis/*chemistry/pathogenicity/*physiology, cdc42 GTP-Binding Protein/analysis/genetics/physiology, rac1 GTP-Binding Protein/analysis/genetics/physiology, rhoA GTP-Binding Protein/analysis/genetics/physiology
Identifiers
urn:nbn:se:umu:diva-16693 (URN)10.1111/j.1462-5822.2005.00684.x (DOI)16681842 (PubMedID)
Available from: 2007-10-09 Created: 2007-10-09 Last updated: 2010-11-22Bibliographically approved
2. Regulation of Yersinia Yop-effector delivery by translocated YopE
Open this publication in new window or tab >>Regulation of Yersinia Yop-effector delivery by translocated YopE
Show others...
2008 (English)In: International Journal of Medical Microbiology, ISSN 1438-4221, E-ISSN 1618-0607, Vol. 298, no 3-4, 183-192 p.Article in journal (Refereed) Published
Abstract [en]

The bacterial pathogen Yersinia pseudotuberculosis uses a type III secretion (T3S) system to translocate Yop effectors into eukaryotic cells. Effectors are thought to gain access to the cytosol via pores formed in the host cell plasma membrane. Translocated YopE can modulate this pore formation through its GTPase-activating protein (GAP) activity. In this study, we analysed the role of translocated YopE and all the other known Yop effectors in the regulation of effector translocation. Elevated levels of Yop effector translocation into HeLa cells occurred by YopE-defective strains, but not those defective for other Yop effectors. Only Yersinia devoid of YopK exhibits a similar hyper-translocation phenotype. Since both yopK and yopE mutants also failed to down-regulate Yop synthesis in the presence of eukaryotic cells, these data imply that translocated YopE specifically regulates subsequent effector translocation by Yersinia through at least one mechanism that involves YopK. We suggest that the GAP activity of YopE might be working as an intra-cellular probe measuring the amount of protein translocated by Yersinia during infection. This may be a general feature of T3S-associated GAP proteins, since two homologues from Pseudomonas aeruginosa, exoenzyme S (ExoS) and exoenzyme T (ExoT), can complement the hyper-translocation phenotypes of the yopE GAP mutant.

Place, publisher, year, edition, pages
Elsevier, 2008
Keyword
YopE; GAP activity; ExoS; ExoT, feedback inhibition, translocation, type III secretion
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:umu:diva-20424 (URN)10.1016/j.ijmm.2007.04.007 (DOI)17597003 (PubMedID)
Available from: 2009-03-19 Created: 2009-03-19 Last updated: 2015-06-26
3. The membrane localization domain is required for intracellular localization and autoregulation of YopE in Yersinia pseudotuberculosis.
Open this publication in new window or tab >>The membrane localization domain is required for intracellular localization and autoregulation of YopE in Yersinia pseudotuberculosis.
Show others...
2009 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 77, no 11, 4740-4749 p.Article in journal (Refereed) Published
Abstract [en]

Recent work has shown that a domain of YopE of Yersinia pseudotuberculosis ranging from amino acids 54 to 75 (R. Krall, Y. Zhang, and J. T. Barbieri, J. Biol. Chem. 279:2747-2753, 2004) is required for proper localization of YopE after ectopic expression in eukaryotic cells. This domain, called the membrane localization domain (MLD), has not been extensively studied in Yersinia. Therefore, an in cis MLD deletion mutant of YopE was created in Y. pseudotuberculosis. The mutant was found to secrete and translocate YopE at wild-type levels. However, the mutant was defective in the autoregulation of YopE expression after the infection of HeLa cells. Although the mutant translocated YopE at wild-type levels, it showed a delayed HeLa cell cytotoxicity. This delay was not caused by a change in GTPase activating protein (GAP) activity, since the mutant showed wild-type YopE GAP activity toward Rac1 and RhoA. The MLD mutant displayed a changed intracellular localization pattern of YopE in HeLa cells after infection, and the YopEDeltaMLD protein was found to be dispersed within the whole cell, including the nucleus. In contrast, wild-type YopE was found to localize to the perinuclear region of the cell and was not found in the nucleus. In addition, the yopEDeltaMLD mutant was avirulent. Our results suggest that YopE must target proteins other than RhoA and Rac1 and that the MLD is required for the proper targeting and hence virulence of YopE during infection. Our results raise the question whether YopE is a regulatory protein or a "true" virulence effector protein.

Place, publisher, year, edition, pages
American Society for Microbiology, 2009
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:umu:diva-32212 (URN)10.1128/IAI.00333-09 (DOI)19687205 (PubMedID)
Available from: 2010-03-03 Created: 2010-03-03 Last updated: 2010-11-22Bibliographically approved
4. Fish scale keratocytes constitute a sensitive model system for YopE mediated phenotypes in Yersinia pseudotuberculosis infections
Open this publication in new window or tab >>Fish scale keratocytes constitute a sensitive model system for YopE mediated phenotypes in Yersinia pseudotuberculosis infections
2010 (English)Manuscript (preprint) (Other academic)
Abstract [en]

The bacterial pathogen Yersinia pseudotuberculosis employs a type 3 secretion system (T3SS) to deliver virulence associated effectors directly into the host cell. Four of the translocated effectors affect the actin cytoskeleton of the cell, demonstrating the importance of inducing actin rearrangements for the establishment of a successful Yersinia infection. To further examine the role of the effectors in actin targeting, we have established a novel model system using fish scale keratocytes. Fish scale keratocytes are rapidly migrating cells present on the surface of teleost skin. The keratocytes are part of the quick wound repair mechanism of fish and are also able to internalize bacteria. The cell consists of a large extended lamellipodium, a two-dimensional actin network regulated by RhoGTPases.                                         By using live cell microscopy, we identified that wild-type Y. pseudotuberculosis caused a cytotoxic effect towards the keratocytes already within 10 minutes of infection. In contrast, a bacterial strain lacking the T3SS was rapidly internalized by the keratocytes. Further, YopE was found to be the sole effector responsible for this cytotoxic effect. YopE inactivates multiple small RhoGTPases via its GAP activity (GTPase activating protein) to induce cytotoxicity in a wide variety of cell types, including HeLa cells. Several domains of YopE are important for proper GAP function. When a number of earlier isolated point mutants of YopE were examined, none of the mutants could induce cytotoxicity towards keratocytes. Thus, this finding is in sharp contrast to earlier observations in HeLa cells, where all but one mutation caused cytotoxicity. In conclusion, the keratocytes appear to be more sensitive towards YopE mediated effects than HeLa cells and would therefore constitute a relevant model system for Yersinia infections for further studies.

Publisher
56 p.
Keyword
Yersinia, infection, fish keratocytes, YopE, cytotoxicity
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-37900 (URN)978-91-7459-100-2 (ISBN)
Available from: 2010-11-19 Created: 2010-11-18 Last updated: 2013-06-12Bibliographically approved

Open Access in DiVA

fulltext(1926 kB)358 downloads
File information
File name FULLTEXT01.pdfFile size 1926 kBChecksum SHA-512
7d5429a4982ec8e88d1941bb78c8bd6abc6db72605090fe23d74b6257628e69103a926b23d85e01bc29728d276ae96e26c84feefe6d13d955dc8b06492d54124
Type fulltextMimetype application/pdf

Authority records BETA

Isaksson, Elin

Search in DiVA

By author/editor
Isaksson, Elin
By organisation
Department of Molecular Biology (Faculty of Science and Technology)
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 358 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 662 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf